Comparative pharmacokinetic study of the main components of cortex fraxini after oral administration in normal and hyperuricemic rats

Published on Aug 1, 2017in Biomedical Chromatography1.728
· DOI :10.1002/BMC.3934
Yinan Wang7
Estimated H-index: 7
(SPU: Shenyang Pharmaceutical University),
Min Zhao8
Estimated H-index: 8
(SPU: Shenyang Pharmaceutical University)
+ 4 AuthorsChunjie Zhao6
Estimated H-index: 6
(SPU: Shenyang Pharmaceutical University)
Cortex Fraxini is an important traditional Chinese herbal medicine used for the treatment of gout and hyperuricemia. An efficient and rapid ultra-performance liquid chromatography mass spectrometry method was developed and validated for simultaneous quantitation of six coumarins (aesculin, fraxin, aesculetin, fraxetin, sopoletin and 7-hydroxycoumarin) in normal and hyperuricemic rats plasma after oral administration of Cortex Fraxini. The method could successfully be applied for pharmacokinetics studies. The pharmacokinetic behavior of six coumarins in normal and hyperuricemia rats plasma was determined. Results showed that, for some of analytes, the pharmacokinetic parameters (AUC0–t, AUC0–∞, Cmax, Tmax and CL) were significantly different between normal and hyperuricemic rats. The different pharmacokinetic parameters might result from renal impairment or a change of metabolic enzymes in the pathological state. The pharmacokinetic study in pathological state could provide more useful information to guide the clinical use of traditional Chinese herbal medicine.
#1Minmin Zhao (Hebei Medical University)H-Index: 6
#2Weijing Ding (Hebei Medical University)H-Index: 6
Last. Shumin JinH-Index: 2
view all 8 authors...
Abstract A high performance liquid chromatography-tandem mass spectrometry (HPLC–MS/MS) method was developed for the simultaneous determination of nine coumarins including aesculin, aesculetin, fraxin, fraxetin, scopoletin, isoscopoletin, 6-hydroxy-7,8-dimethoxy coumarin, 8-hydroxy-6,7-dimethoxy coumarin and umbelliferone in rat plasma using nodakenin as the internal standard (IS). The plasma samples were pretreated by a one-step direct protein precipitation with methanol. The chromatographic se...
10 CitationsSource
#1Yinan Wang (SPU: Shenyang Pharmaceutical University)H-Index: 7
#2Min Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 8
Last. Chunjie Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 6
view all 6 authors...
Abstract Ethnopharmacological relevance Cortex Fraxini (CF) is an important traditional Chinese herbal medicine used for the treatment of gout and hyperuricemia. Aim of the study The aim of this study was to evaluate the anti-hyperuricemic effect of CF on hyperuricemic rats and to investigate its mechanism of action. Materials and methods Metabolomics based on NMR and MS was used to study the therapeutic effect of CF on hyperuricemic rats. Plasma determination of uric acid (UA) showed that CF tr...
21 CitationsSource
#1Yinan Wang (SPU: Shenyang Pharmaceutical University)H-Index: 7
#2Min Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 8
Last. Chunjie Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 5
view all 7 authors...
Abstract Esculin, a coumarin derivative found in Fraxinus rhynchophylla , has been reported to possess multiple biological activities. This present study is designed to investigate the metabolic profile of esculin in vivo based on ultra high performance liquid chromatography coupled to Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) for the first time. After oral administration of esculin (100 mg/kg) for rats, plasma, urine, feces and bile samples were collected to ...
19 CitationsSource
#1Yinan Wang (SPU: Shenyang Pharmaceutical University)H-Index: 7
#2Min Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 8
Last. Chunjie Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 6
view all 5 authors...
Cortex Fraxini is a widely used traditional Chinese medicine (TCM) used for the treatment of gout or hyperuricemia. In this study, a reliable and sensitive ultra-high performance liquid chromatography coupled with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS) method was developed for systematical screen and identification of the metabolic profile in rats after oral administration of Cortex Fraxini. The chromatographic separation was performed on a Universil XB C18...
10 CitationsSource
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A rapid, sensitive and selective liquid chromatography-electrospray ionization-tandem mass spectrometric method was developed and validated for the quantification of scopoletin in rat plasma. After the addition of the internal standard xanthotoxin, plasma samples were pretreated by a simple one-step protein precipitation with acetonitrile-methanol (2:1, v/v). Chromatographic separation was achieved on a Diamonsil ODS chromatography column using gradient elution with the mobile phase consisting o...
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Aims This open label study was conducted to assess the effect of renal impairment (RI) on the pharmacokinetics (PK) of peginterferon lambda-1a (Lambda). Methods Subjects (age 18–75 years, BMI 18–35 kg m–2) were enrolled into one of five renal function groups: normal (n = 12), mild RI (n = 8), moderate RI (n = 8), severe RI (n = 7), end-stage renal disease (ESRD, n = 8) based on estimated glomerular filtration rate (eGFR) calculated using the Modification of Diet in Renal Disease (MDRD) equation....
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#1Antoinette R. Tan (RU: Rutgers University)H-Index: 30
#2John Sarantopoulos (University of Texas Health Science Center at San Antonio)H-Index: 25
Last. Sanjay Goel (Albert Einstein College of Medicine)H-Index: 23
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Purpose To evaluate the effect of renal impairment on eribulin mesylate pharmacokinetics following a single dose in adults with advanced solid tumors.
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The significance of transporters in the disposition, metabolism, and elimination of drugs is well recognized. One gap in our knowledge is a comprehensive understanding of how drug transporters change functionality (their amount and activity) in response to disease and how disease and its inevitable pathology change transporter expression. In this issue of Drug Metabolism and Disposition a series of review and primary research articles are presented to highlight the importance of transporters in ...
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Last. Rosalinde Masereeuw (Radboud University Nijmegen Medical Centre)H-Index: 59
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Abstract Hyperuricemia is related to a variety of pathologies, including chronic kidney disease (CKD). However, the pathophysiological mechanisms underlying disease development are not yet fully elucidated. Here, we studied the effect of hyperuricemia on tryptophan metabolism and the potential role herein of two important uric acid efflux transporters, multidrug resistance protein 4 (MRP4) and breast cancer resistance protein (BCRP). Hyperuricemia was induced in mice by treatment with the uricas...
33 CitationsSource
#1Pai-Feng Hsu (NYMU: National Yang-Ming University)H-Index: 4
#1Pai-Feng Hsu (NYMU: National Yang-Ming University)H-Index: 17
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Hyperuricemia, and its clinical manifestation gout, is a metabolic disease process that has been recognized since the dawn of medical inquiry. Uric acid was hypothesized to be a mediator of cardiovascular disease for period of time. Epidemiological correlations of hyperuricemia with hypertension and cardiovascular events were evident for two centuries’ studies. With recent animal studies shedding light on the causal mechanisms of hypertension, and clinical trials suggesting that urate-lowering t...
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#1Tingting Liu (SPU: Shenyang Pharmaceutical University)H-Index: 1
#2Min Zhao (SPU: Shenyang Pharmaceutical University)H-Index: 8
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Clinically, Wangbi Capsule (WBC) is widely used in the treatment of Rheumatoid arthritis (RA) because of its remarkable therapeutic effect. To reveal the mechanism, a pharmacokinetic-pharmacodynamic (PK-PD) model was developed for the first time to assess the relationship between time-concentration (dose)-effect. Freund's Complete Adjuvant (FCA) was used to induce the Adjuvant-Induced Arthritis (AIA) model. Multi-index were used to evaluate the therapeutic effect and a S-Imax PK-PD model was est...
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