Generation of nephron progenitor cells and kidney organoids from human pluripotent stem cells

This protocol describes how to differentiate human pluripotent stem cells into nephron progenitor cells with subsequent generation of 2D and 3D kidney organoids. A variety of protocols have been developed that demonstrate the capability to differentiate human pluripotent stem cells (hPSCs) into kidney structures. Our goal was to develop a high-efficiency protocol to generate nephron progenitor cells (NPCs) and kidney organoids to facilitate...