AbstractContext Gynostemma pentaphyllum (Thunb.) Makino has been used in traditional medicine for the treatment of hyperlipidaemic with a long history.Objective: The objective of this study was to evaluate the influence of Gynostemma pentaphyllum (GP) and atorvastatin on amino acids from the plasma and liver tissue of hyperlipidaemic rats.Materials and methods The rats were fed a high-fat diet continuously for 11 weeks for the construction of hyperlipidaemic model. The hyperlipidaemic rats were treated with Gynostemma pentaphyllum (120 mg/kg) and atorvastatin (1.8 mg/kg) for 4 weeks, and the rats were intragastric administration one time every day. Chromatographic separation was performed on a Shim-pack XR-ODSIII C18 analytical column (75 mm × 2.0 mm i.d., 1.6 μm, Shmadazu Corp., Tokyo, Japan). The biomarkers of amino acids were identified by principal component analysis (PCA) and partial least squares discriminant analysis (PLS-DA).Results After feeding with a high-fat diet, the TC and LDL-C values of th...
#2Xian Zhou(Soochow University (Suzhou))H-Index: 2
Last. Chang Wang(Soochow University (Suzhou))H-Index: 2
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Rapid radiation injury early triage after a radiological or nuclear exposure is vital for treatment of a large number of wounded people. Owing to the high-throughput analysis and minimally invasive nature of sample collection, radiation metabolomics has been recently applied to radiation damage research. In the present study, exploring the feasibility of estimating the acute radiation injury for early triage by means of urinary amino acid target analysis was attempted using a high performance li...
#1J.D. Sharer(UAB: University of Alabama at Birmingham)H-Index: 1
Amino acids function as the building blocks of proteins, as energy sources, and as precursors for a number of different biological molecules. The chemical properties of amino acid side chains largely determine the structural and functional characteristics of proteins. Multiple metabolic pathways exist for the synthesis and catabolism of different amino acids. Mutational impairment of these pathways can cause a number of different inherited diseases that often cause significant neurological dysfu...
Last. Chunjie Zhao(SPU: Shenyang Pharmaceutical University)H-Index: 6
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Gynostemma pentaphyllum (GP) is widely used for the treatment of diseases such as hyperlipidemia, fatty liver and obesity in China, and atorvastatin is broadly used as an anti-hyperlipidemia drug. This research focuses on the plasma and liver metabolites in the following four groups of rats: control, a hyperlipidemia model, a hyperlipidemia model treated with GP and a hyperlipidemia model treated with atorvastatin. Using 1H-NMR-based metabonomics, we elucidated the therapeutic mechanisms of GP a...
Last. Chang Wang(Soochow University (Taiwan))H-Index: 1
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Rapid radiation injury early triage for large-scale people after radiation exposure is vital for limited medical resources allocation and early treatment of a large number of wounded after a nuclear accident. Owing to the high-throughput analysis and minimally invasive of collection sample, radiation metabolomics has been recently applied to radiation damage researches. Here, exploration the feasibility of estimating the acute radiation injury early triage by means of plasma amino acid target an...
Aims. To evaluate the effect of the traditional Vietnamese herb Gynostemma pentaphyllum tea on insulin sensitivity in drug-naive type 2 diabetic patients. Methods. Patients received GP or placebo tea 6 g daily for four weeks and vice versa with a 2-week wash-out period. At the end of each period, a somatostatin-insulin-glucose infusion test (SIGIT) was performed to evaluate the insulin sensitivity. Fasting plasma glucose (FPG), , and oral glucose tolerance tests and insulin levels were measured ...
Last. Wen-Cai Ye(JNU: Jinan University)H-Index: 46
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In the present study, the protective effects of gypenosides from Gynostemma pentaphyllum on fatty liver disease (FLD) were examined in rats treated with high fat and cholesterol diet and alcohol. Male SD rats were divided into seven groups: control, model, lovastatin, silymarin, gypenosides high-, medium- and low-treatment groups. The latter 6 groups were fed high-fat and cholesterol diet and administered alcohol intragastricly once a day. Body weight was measured every week for 10 weeks, and th...
We present a method for the qualitative and quantitative study of transient metabolic flux of phage infection at the molecular level. The method is based on statistical total correlation spectroscopy (STOCSY) and partial least squares discriminant analysis (PLS-DA) applied to nuclear magnetic resonance (NMR) metabonomic data sets. An algorithm for this type of study is developed and demonstrated. The method has been implemented on 1H NMR data sets of growth media in planktonic cultures of Pseudo...
Last. Yu-Qing Zhao(SPU: Shenyang Pharmaceutical University)H-Index: 2
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Abstract Five novel triterpenes were isolated from hydrolyzate of total saponins from Gynostemma pentaphyllum and identified as gypensapogenin A ( 1 ), gypensapogenin B ( 2 ), gypensapogenin C ( 3 ), 3-O- β - d -glucopyranosyl-gypensapogenin D ( 4 ) and gypensapogenin D ( 5 ), two of which ( 1 and 2 ) possess unprecedented ring A. The cyclization of the side chains-3 formed five-membered cyclic ketone rings similar to ring E in 1. The 21-oic acid-21, 23-lactone was present in the side chains of ...
Partial least squares (PLS) was not originally designed as a tool for statistical discrimination. In spite of this, applied scientists routinely use PLS for classification and there is substantial empirical evidence to suggest that it performs well in that role. The interesting question is: why can a procedure that is principally designed for overdetermined regression problems locate and emphasize group structure? Using PLS in this manner has heurestic support owing to the relationship between P...
#1Wenqian Zhang(MUST: Macau University of Science and Technology)H-Index: 3
#2Tingting Zhao(MUST: Macau University of Science and Technology)H-Index: 22
Last. Youhua Xu(MUST: Macau University of Science and Technology)H-Index: 18
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: Aging has become a worldwide problem. During this process, incidence of related diseases such as diabetes and atherosclerosis increases dramatically. Studies within most recent two decades suggest pivotal role of advanced glycation end products (AGEs) in aging process. This review aims to systemically summarize effects and potential mechanism of Chinese Medicines on inhibiting AGEs-related aging diseases.
Amino acids are indispensable components of living organisms. The high amino acid content in Nitraria tangutorum Bobr. fruit distinguishes it from other berry plants and is of great significance to its nutritional value. Herein, using 10-ethyl-acridine-3-sulfonyl chloride as a fluorescent pre-column labeling reagent, a method for the efficient and rapid determination of amino acid content in N. tangutorum by pre-column fluorescence derivatization and on-line mass spectrometry was established and...
: Hypercholesterolemia is a major risk factor for the initiation and development of nonalcoholic fatty liver disease and atherosclerosis. The present study evaluated the hypocholesterolemic effect of virgin avocado oil (VAO) using urinary metabolomic method. Male Sprague-Dawley rats were fed high-cholesterol diet for four weeks to induce hypercholesterolemia. After confirming the establishment of hypercholesterolemia model, the VAO (450 and 900 mg·kg-1 ·day-1 ) and simvastatin (10 mg·kg-1 ·day-1...
#1Fei Xu(Nanjing University of Chinese Medicine)H-Index: 5
#2Cai Lu(Nanjing University of Chinese Medicine)H-Index: 3
Last. You Min(Nanjing University of Chinese Medicine)H-Index: 2
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Abstract Studies on the lipid-regulating effects of alisol compounds are reported that include alisol B, alisol A 24-acetate (24A), alisol A and an alisol B - 24A - alisol A mixture (content ratio = 1:1:1). The effects on the activity of lipoprotein lipase (LPL), a key lipid-modulating enzyme, were studied to investigate the molecular mechanism of lipid-regulating activity of alisols. The effects of alisols on regulating blood lipids and the activities of LPL were determined using a reagent kit ...
Last. Makoto Tsunoda(UTokyo: University of Tokyo)H-Index: 29
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Abstract Amino acids are widely distributed in biological fluids and involved in many biological processes, such as the synthesis of proteins, fatty acids, and ketone bodies. The altered levels of amino acids in biological fluids have been found to be closely related to several diseases, such as type 2 diabetes, kidney disease, liver disease, and cancer. Therefore, the development of analytical methods to measure amino acid concentrations in biological samples can contribute to research on the p...
#1Nicolas Drouin(UNIL: University of Lausanne)H-Index: 8
#2Serge Rudaz(UNIL: University of Lausanne)H-Index: 73
Last. Julie Schappler(UNIL: University of Lausanne)H-Index: 29
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Sample preparation is a primary step of any bioanalytical workflow, especially in metabolomics analysis where maximum information has to be obtained without spoiling the analytical instrument. Because of their biological implication, highly polar metabolites, such as amino acids, nucleobases, and catecholamines seem to attract growing interest in the field of comprehensive metabolomics analysis although their extraction from the matrix remains a real challenge. In this paper, we discuss about th...