Microarray analysis identified differentially expressed genes in keratocytes from keratoconus patients

Purpose. To identify differentially expressed genes in human keratoconus keratocyte by cDNA microarray. Methods. Normal and keratoconic cornea were cultured for keratocytes. RNA was extracted. cDNA probe labeled with fluorescence dye was made from Poly A+ RNA, hybridized with microarray slide, containing 164 human apoptosis genes. Signal intensity was measured. Expression ratio between keratoconus and normal was determined using ImaGene Ver.3.0....