Açaí (Euterpe oleracea Mart.) Modulates Oxidative Stress Resistance in Caenorhabditis elegans by Direct and Indirect Mechanisms

Published on Mar 3, 2014in PLOS ONE2.74
· DOI :10.1371/JOURNAL.PONE.0089933
Larissa de Freitas Bonomo4
Estimated H-index: 4
(UFOP: Universidade Federal de Ouro Preto),
David Nunes Silva1
Estimated H-index: 1
(UFOP: Universidade Federal de Ouro Preto)
+ 13 AuthorsRiva de Paula Oliveira19
Estimated H-index: 19
(UFOP: Universidade Federal de Ouro Preto)
Acai (Euterpe oleracea Mart.) has recently emerged as a promising source of natural antioxidants. Despite its claimed pharmacological and nutraceutical value, studies regarding the effects of acai in vivo are limited. In this study, we use the Caenorhabditis elegans model to evaluate the in vivo antioxidant properties of acai on an organismal level and to examine its mechanism of action. Supplementation with acai aqueous extract (AAE) increased both oxidative and osmotic stress resistance independently of any effect on reproduction and development. AAE suppressed bacterial growth, but this antimicrobial property did not influence stress resistance. AAE-increased stress resistance was correlated with reduced ROS production, the prevention of sulfhydryl (SH) level reduction and gcs-1 activation under oxidative stress conditions. Our mechanistic studies indicated that AAE promotes oxidative stress resistance by acting through DAF-16 and the osmotic stress response pathway OSR-1/UNC-43/SEK-1. Finally, AAE increased polyglutamine protein aggregation and decreased proteasome activity. Our findings suggest that natural compounds available in AAE can improve the antioxidant status of a whole organism under certain conditions by direct and indirect mechanisms.
Figures & Tables
📖 Papers frequently viewed together
1 Author (Sydney Brenner)
10.2k Citations
152 Citations
442 Citations
#1Atsushi IshikadoH-Index: 16
#2Yoko SonoH-Index: 3
Last. Taketoshi MakinoH-Index: 13
view all 9 authors...
Willow bark extract (WBE) is listed in the European Pharmacopoeia and has been traditionally used for treating fever, pain, and inflammation. Recent studies have demonstrated its clinical usefulness. This study investigated the antioxidative effects of WBE in human umbilical vein endothelial cells (HUVECs) and Caenorhabditis elegans. WBE prevented oxidative-stress-induced cytotoxicity of HUVECs and death of C. elegans. WBE dose-dependently increased mRNA and protein expression levels of the nucl...
36 CitationsSource
#1Sujay Guha (Clemson University)H-Index: 5
#2Min Cao (Clemson University)H-Index: 18
Last. Yuqing Dong (Clemson University)H-Index: 8
view all 6 authors...
Nutraceuticals are known to have numerous health and disease preventing properties. Recent studies suggest that extracts containing cranberry may have anti-aging benefits. However, little is known about whether and how cranberry by itself promotes longevity and healthspan in any organism. Here we examined the effect of a cranberry only extract on lifespan and healthspan in Caenorhabditis elegans. Supplementation of the diet with cranberry extract (CBE) increased the lifespan in C. elegans in a c...
48 CitationsSource
#1Jian Song (MOE: Chinese Ministry of Education)H-Index: 1
#2Mengyao Zhao (MOE: Chinese Ministry of Education)H-Index: 8
Last. Fang Chen (MOE: Chinese Ministry of Education)H-Index: 19
view all 6 authors...
Abstract Acrylamide (AA) occurs in many cooked starchy foods and has caused widespread concern as a possible carcinogen. In the present study, we investigate the intervention of AA toxicity in MDA-MB-231 cells pretreated with cyanidin-3-glucoside (Cy-3-glu). Compared to the cells treated with AA, Cy-3-glu significantly inhibited AA-induced cytotoxicity, reduced reactive oxygen species (ROS) generation, recovered glutathione (GSH) depletion and decreased the activities of glutathione peroxidase (...
49 CitationsSource
#1Christian Büchter (MLU: Martin Luther University of Halle-Wittenberg)H-Index: 8
#2Daniela Ackermann (MLU: Martin Luther University of Halle-Wittenberg)H-Index: 1
Last. Wim Wätjen (MLU: Martin Luther University of Halle-Wittenberg)H-Index: 28
view all 8 authors...
Myricetin is a naturally occurring flavonol found in many plant based food sources. It increases the lifespan of Caenorhabditis elegans, but the molecular mechanisms are not yet fully understood. We have investigated the impact of this flavonoid on the transcription factors DAF-16 (C. elegans FoxO homologue) and SKN-1 (Nrf2 homologue), which have crucial functions in the regulation of ageing. Myricetin is rapidly assimilated by the nematode, causes a nuclear translocation of DAF-16 but not of SK...
70 CitationsSource
#1André Gordon (University of Bonn)H-Index: 6
#2Ana Paula Gil Cruz (UFRJ: Federal University of Rio de Janeiro)H-Index: 4
Last. Friedhelm Marx (University of Bonn)H-Index: 17
view all 10 authors...
Abstract Consumption of acai fruits has been linked to positive health effects due to its phenolic content and nutritive value. The objective of this study was to characterize acai fruits chemically and to determine the antioxidant capacity at three different maturity stages. With the exception of fat, amounts of macronutrients, minerals and titratable acids decreased during the ripening process. The same trend was observed for most of the phenolic constituents identified by HPLC–ESI-MS/MS. A co...
95 CitationsSource
#1Pablo LiedoH-Index: 37
#2James R. Carey (UC Davis: University of California, Davis)H-Index: 45
Last. Sige Zou (NIH: National Institutes of Health)H-Index: 24
view all 4 authors...
Macronutrient balance is a critical contributor in modulating lifespan and health. Consumption of diets rich in fruits and vegetables provides numerous health benefits. The interactions among macronutrients and botanicals and how they influence aging and health remain elusive. Here we employed a nutritional geometry approach to investigate the interplay among dietary fat, sugar, protein and antioxidant- and polyphenolic-rich freeze-dried acai pulp in modulating lifespan and reproductive output i...
13 CitationsSource
#1Yeu-Ching Shi (NTU: National Taiwan University)H-Index: 12
#2Chan-Wei Yu (NTU: National Taiwan University)H-Index: 13
Last. Tzu-Ming Pan (NTU: National Taiwan University)H-Index: 43
view all 4 authors...
Background Monascus-fermented products are mentioned in an ancient Chinese pharmacopoeia of medicinal food and herbs. Monascus-fermented products offer valuable therapeutic benefits and have been extensively used in East Asia for several centuries. Several biological activities of Monascus-fermented products were recently described, and the extract of Monascus-fermented products showed strong antioxidant activity of scavenging DPPH radicals. To evaluate whether Monascus-fermented dioscorea produ...
15 CitationsSource
#1Amadeo Gironés-Vilaplana (CSIC: Spanish National Research Council)H-Index: 17
#2Patrícia ValentãoH-Index: 56
Last. Paula B. AndradeH-Index: 60
view all 6 authors...
Following previous research on lemon juice enriched with berries, the aim of this work was to design new blends based on lemon juice mixed with different edible berries of exotic and national origin: maqui (Aristotelia chilensis (Molina) Stuntz), acai (Euterpe oleracea Mart.), and blackthorn (Prunus spinosa L.). The phytochemical characterization of controls and blends was performed by HPLC-DAD-ESI/MSn. Their antioxidant capacity against DPPH, superoxide, and hydroxyl radicals and hypochlorous a...
36 CitationsSource
#1Susana González-Manzano (University of Salamanca)H-Index: 28
Last. Celestino Santos-BuelgaH-Index: 68
view all 7 authors...
The aim of this work was to examine the mechanisms involved in the in vivo antioxidant effects of epicatechin (EC), a major flavonoid in the human diet. The influence of EC in different oxidative biomarkers (reactive oxygen species (ROS) production, intracellular glutathione, activity of catalase (CAT), glutathione peroxidase (GPx), and superoxide dismutase (SOD)) was studied in the model organism Caenorhabditis elegans. Under thermal stress condition, exposure of the worms (wild type N2 strains...
32 CitationsSource
#1Gregor Grünz (TUM: Technische Universität München)H-Index: 2
#2Kerstin Haas (TUM: Technische Universität München)H-Index: 1
Last. Britta Spanier (TUM: Technische Universität München)H-Index: 14
view all 7 authors...
Abstract Various studies have demonstrated longevity effects of flavonoids, a major sub-group of plant polyphenolic compounds, in Caenorhabditis elegans . To better understand their structure–activity relationship in vivo we have used a comparative approach by exposing C. elegans to the structurally related flavonoids myricetin, quercetin, kaempferol and naringenin, and assessed their impact on lifespan and on putative modes of action. The bioavailability of the tested flavonoids was demonstrate...
90 CitationsSource
Cited By43
#1Talita Alves Faria Martins Magalhães (UFOP: Universidade Federal de Ouro Preto)H-Index: 1
#2Melina Oliveira de Souza (UFOP: Universidade Federal de Ouro Preto)H-Index: 5
Last. Joana Ferreira do Amaral (UFOP: Universidade Federal de Ouro Preto)H-Index: 5
view all 7 authors...
Intestinal mucositis (IM) caused by antineoplastic chemotherapy is characterized by an important inflammatory process, which may compromise ongoing treatment. Our study aimed to investigate the effect of Acai (Euterpe oleracea Martius) on the antioxidant response in BALB/c mice pretreated with Acai pulp (200 g/kg) for 14 day. A group of animals receiving a single intraperitoneal injection of 5-FU (200 mg/kg) were euthanized on day three (D3) or seven (D7) after administration, the distal jejunum...
1 CitationsSource
Caffeic and dihydrocaffeic acid are relevant microbial catabolites, being described as products from the degradation of different phenolic compounds i.e., hydroxycinnamoyl derivatives, anthocyanins or flavonols. Furthermore, caffeic acid is found both in free and esterified forms in many fruits and in high concentrations in coffee. These phenolic acids may be responsible for a part of the bioactivity associated with the intake of phenolic compounds. With the aim of progressing in the knowledge o...
#1Victor Neiva Lavorato (UFOP: Universidade Federal de Ouro Preto)H-Index: 1
Last. Antônio José NataliH-Index: 18
view all 10 authors...
Abstract This study evaluated the effect of aerobic exercise training (AET) and supplementation with acai on cardiac structure and function in rats submitted to a high-fat diet. Two-month old Fischer male rats were divided into 5 groups: Control (C), High-fat Diet (H), High-fat Diet + Acai (HA), High-fat Diet + AET (HT), High-fat Diet + Acai + AET (HAT). The high-fat diet had 21.8% lard and 1% cholesterol (H and HT), or supplemented with 1% lyophilized acai pulp (HA and HAT). The HT and HAT grou...
#1Débora P. Moraes (UFSM: Universidade Federal de Santa Maria)H-Index: 1
#2Marina Lopes Machado (UFSM: Universidade Federal de Santa Maria)H-Index: 6
Last. Milene Teixeira Barcia (UFSM: Universidade Federal de Santa Maria)H-Index: 3
view all 12 authors...
#1T.F.S. Curimbaba (UNESP: Sao Paulo State University)H-Index: 1
#2L.D. Almeida-Junior (UNESP: Sao Paulo State University)H-Index: 1
Last. L.C. Di Stasi (UNESP: Sao Paulo State University)H-Index: 8
view all 6 authors...
Abstract Dietary products with prebiotic and antioxidant properties may be used to protect against chronic diseases, including inflammatory bowel diseases. Assai berry, burity palm and cupuassu from the Amazon are functional foods because of their high level of dietary fiber and polyphenols, which have an important role in the prevention of inflammatory processes. Their activity as a dietary supplement was measured using fruit pulp flours in a trinitrobenzenesulphonic acid model of intestinal in...
#1Natasha LeiteH-Index: 1
Last. Edson Lucas dos SantosH-Index: 20
view all 10 authors...
Fruits are sources of bioactive compounds that are responsible for several biological activities. Therefore, this study aimed to identify the chemical composition of the pulp of the Brazilian Savanna fruit Dipteryx alata; evaluate its toxic effects, influence on the life expectancy of the nematode Caenorhabditis elegans, and its antioxidant activities in vitro and in vivo; and describe the mechanisms involved. The chemical compounds identified include phenols, terpenes, fatty acid derivatives, v...
#1Begoña Ayuda-Durán (University of Salamanca)H-Index: 7
#2Susana González-Manzano (University of Salamanca)H-Index: 28
Last. Celestino Santos-Buelga (University of Salamanca)H-Index: 68
view all 4 authors...
The nematode Caernohabditis elegans was introduced as a model organism in biological research by Sydney Brenner in the 1970s. Since then, it has been increasingly used for investigating processes such as ageing, oxidative stress, neurodegeneration, or inflammation, for which there is a high degree of homology between C. elegans and human pathways, so that the worm offers promising possibilities to study mechanisms of action and effects of phytochemicals of foods and plants. In this paper, the ge...
4 CitationsSource
#1Lívia Gabrielle Maciel Sales (UFC: Federal University of Ceará)H-Index: 1
#2Gizele Almada Cruz (UFC: Federal University of Ceará)H-Index: 1
Last. Juliane Döering Gasparin Carvalho (UFC: Federal University of Ceará)H-Index: 3
view all 6 authors...
O Kefir e um leite fermentado que pode apresentar propriedades funcionais, dentre as quais estao a regulacao intestinal e aumento da resistencia imunologica. A adicao de frutas ao Kefir agrega valor nutricional e sensorial ao produto. Esta pesquisa objetivou produzir Kefir adicionado de polpa de acai e avaliar suas caracteristicas fisico-quimicas, sensoriais e sua estabilidade por 45 dias. O Kefir foi elaborado a partir de leite fermentado por meio dos graos de Kefir com diferentes concentracoes...
#1Shital Doshi (St. Xavier's College-Autonomous, Mumbai)
#2Vincent Braganza (St. Xavier's College-Autonomous, Mumbai)
Abstract Background Argyreia boseana Santapau and Patel commonly known as Kumbharao belongs to the family of Convolvulaceae. The plant is rare in distribution and found chiefly in the dediapada region of Gujarat. Traditionally it is used by the tribals of south Gujarat forest region in the treatment of various diseases of the nervous system. In order to study the scientific basis of the plants effect we set out to investigate the effect of the plant on ageing organisms and used Caenorhabditis el...
#1Irene ZorraquínH-Index: 1
#2Eva Sánchez-Hernández (University of Salamanca)H-Index: 2
Last. Begoña BartoloméH-Index: 50
view all 8 authors...
Interactions between polyphenols and gut microbiota are indeed a major issue of current interest in food science research. Knowledge in this subject is progressing as the experimental procedures and analysis techniques do. The aim of this paper is to critically review the more leading-edge approaches that have been applied so far in the study of the interactions between grape/wine polyphenols and gut microbiota. This is the case of in vitro dynamic gastrointestinal simulation models that try to ...
4 CitationsSource