The frpB1 gene of Helicobacter pylori is regulated by iron and encodes a membrane protein capable of binding haem and haemoglobin

Published on Mar 23, 2012in FEBS Letters3.057
· DOI :10.1016/J.FEBSLET.2012.02.015
Miguel Angel Carrizo-Chávez1
Estimated H-index: 1
(UACM: Universidad Autónoma de la Ciudad de México),
Areli Cruz-Castañeda4
Estimated H-index: 4
(UACM: Universidad Autónoma de la Ciudad de México),
José de Jesús Olivares-Trejo13
Estimated H-index: 13
(UACM: Universidad Autónoma de la Ciudad de México)
Sources
Abstract
FrpB1 is a novel membrane protein of Helicobacter pylori that is capable of binding both haem and haemoglobin but consistently shows more affinity for haem. The mRNA levels of frpB1 were repressed by iron and lightly modulated by haem or haemoglobin. The overexpression of the frpB1 gene supported cellular growth when haem or haemoglobin were supplied as the only iron source. Three-dimensional modelling revealed the presence of motifs necessary to bind either haem or haemoglobin. Our overall results support the idea that FrpB1 is a membrane protein of H. pylori that allows this pathogen to survive in the human stomach.
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