Glucose tolerant and thermophilic ?-glucosidases from yeasts

Forty-eight yeast strains belonging to the genera Candida, Debaryomyces, Kluyveromyces and Pichia (obtained from the ARS Culture Collection, Peoria, IL) were screened for production of extracellular glucose tolerant and thermophilic β-glucosidase activity using p-nitrophenyl-β-D-glucoside as substrate. Enzymes from 15 yeast strains showed very high glucose tolerance (<50 % inhibition at 30 %, w/v glucose). The optimal temperatures and pH for...