Characterization of autoantibodies from patients with Goodpasture's disease using a resonant mirror biosenso

SUMMARY Goodpasture's disease is characterized by the binding of IgG autoantibodies to the glomerular basement membrane, leading to glomerular inflammation. The autoantigen has been identified as the noncollagenous domain of the α3 chain of type IV collagen (α3(IV)NC1). We have used the IAsys resonant mirror biosensor to analyse the extent and affinity of binding of anti-GBM antibodies from sera of patients to purified α3(IV) NC1. α3(IV) NC1...