Measurement of platelet fibrinogen binding and p-selectin expression by flow cytometry in patients with cerebral infarction.

Published on Nov 1, 2001in Thrombosis Research3.944
路 DOI :10.1016/S0049-3848(01)00350-4
Masako Yamazaki10
Estimated H-index: 10
,
Shinichiro Uchiyama51
Estimated H-index: 51
,
Makoto Iwata31
Estimated H-index: 31
Sources
Abstract
We determined the percentages of fibrinogen-bound and P-selectin-expressed platelets in whole blood using flow cytometry in 254 patients with various cerebral infarction subtypes, as well as in 30 age-matched controls. Patients with atherothrombotic stroke showed significant increases in both fibrinogen-bound and P-selectin-expressed platelets. Patients with lacunar stroke also showed significant increases in both of them, but the percentage of P-selectin expression was significantly lower than that in atherothrombotic stroke. Patients with cardioembolic stroke showed a significant increase in P-selectin-expressed platelets without any increase in fibrinogen-bound platelets. Platelet fibrinogen binding and P-selectin expression were significantly lower in patients treated with ticlopidine but not with aspirin than in those not treated with any antiplatelet agent, and were lowest in those treated with both ticlopidine and aspirin. Our findings suggest that expression of adhesion molecules on platelet membrane surface differs among the patients with subtypes of ischemic stroke and differs among the types of antiplatelet regimens.
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#1H. J. M. BarnettH-Index: 1
Section I: Pathophysiology of Stroke. Epidemiology of Stroke. Atherosclerosis. Vascular Biology of Cerebral Arteries. Neurochemistry and Molecular Biology. Pathophysiology of White Matter Anoxic/Ischemic Injury. Positron Emission Tomography. Functional Magnetic Resonance Imaging. Pathology. Basic Science of Fibrinolysis: Coronary and Cerebral Thrombolysis. Section Ii: Diagnostic Studies for Stroke. Overview of Laboratory Studies. Computed Tomography Scanning. Magnetic Resonance Scanning. Cerebra...
#1Armin J. Grau (Heidelberg University)H-Index: 49
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Determination of circulating activated platelets may be helpful to estimate the prognosis and to stratify therapies in arterial vascular disorders including stroke. We used flow cytometry and phase contrast microscopy to study whether the fraction of platelets expressing p-selectin and CD63 and the fraction of platelets with shape change are increased in patients with acute and previous cerebrovascular ischemia. The proportion of platelets expressing activation dependent antigens was higher in p...
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: Alterations of platelet, coagulation, and fibrinolysis markers were investigated to determine the indications for antithrombotic therapy in patients with different clinical categories of acute cerebral infarction. Marked platelet activation was observed in platelet function tests, including measurements of platelet-specific proteins and platelet survival, platelet scintigraphy in the brain, and platelet fibrinogen binding assay, in patients with atherothrombotic stroke. Among patients with ath...
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: Platelet activation plays an important role in the pathomechanisms of arterial vascular disorders including stroke, peripheral arterial disease (PAD), and myocardial infarction. Circulating activated platelets may be useful markers of local thrombotic events occurring in these diseases. Using flow cytometry circulating activated platelets can be detected by determining: 1. the platelets' shape change on the basis of the different light scatter properties of discocytes and spherocytes, 2. the e...
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