Measurement of platelet fibrinogen binding and p-selectin expression by flow cytometry in patients with cerebral infarction.

Published on Nov 1, 2001in Thrombosis Research3.944
· DOI :10.1016/S0049-3848(01)00350-4
Masako Yamazaki10
Estimated H-index: 10
 , Shinichiro Uchiyama51
Estimated H-index: 51
 , Makoto Iwata31
Estimated H-index: 31
Sources
Abstract
We determined the percentages of fibrinogen-bound and P-selectin-expressed platelets in whole blood using flow cytometry in 254 patients with various cerebral infarction subtypes, as well as in 30 age-matched controls. Patients with atherothrombotic stroke showed significant increases in both fibrinogen-bound and P-selectin-expressed platelets. Patients with lacunar stroke also showed significant increases in both of them, but the percentage of P-selectin expression was significantly lower than that in atherothrombotic stroke. Patients with cardioembolic stroke showed a significant increase in P-selectin-expressed platelets without any increase in fibrinogen-bound platelets. Platelet fibrinogen binding and P-selectin expression were significantly lower in patients treated with ticlopidine but not with aspirin than in those not treated with any antiplatelet agent, and were lowest in those treated with both ticlopidine and aspirin. Our findings suggest that expression of adhesion molecules on platelet membrane surface differs among the patients with subtypes of ischemic stroke and differs among the types of antiplatelet regimens.
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#1H. J. M. BarnettH-Index: 1
Section I: Pathophysiology of Stroke. Epidemiology of Stroke. Atherosclerosis. Vascular Biology of Cerebral Arteries. Neurochemistry and Molecular Biology. Pathophysiology of White Matter Anoxic/Ischemic Injury. Positron Emission Tomography. Functional Magnetic Resonance Imaging. Pathology. Basic Science of Fibrinolysis: Coronary and Cerebral Thrombolysis. Section Ii: Diagnostic Studies for Stroke. Overview of Laboratory Studies. Computed Tomography Scanning. Magnetic Resonance Scanning. Cerebra...
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#1Armin J. Grau (Heidelberg University)H-Index: 49
#2Andreas RufH-Index: 17
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Determination of circulating activated platelets may be helpful to estimate the prognosis and to stratify therapies in arterial vascular disorders including stroke. We used flow cytometry and phase contrast microscopy to study whether the fraction of platelets expressing p-selectin and CD63 and the fraction of platelets with shape change are increased in patients with acute and previous cerebrovascular ischemia. The proportion of platelets expressing activation dependent antigens was higher in p...
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Jun 1, 1997·Journal of the American College of Cardiology24.09
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#2Meinrad Gawaz (LMU: Ludwig Maximilian University of Munich)H-Index: 96
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Abstract Objectives. This study sought to investigate the contribution of ticlopidine to the inhibition of platelet activation after coronary stent placement. Background. After coronary stenting, antiplatelet therapy with aspirin and ticlopidine improves stent patency compared with anticoagulation. However, the specific role of ticlopidine has not been elucidated. Methods. After successful coronary stent placement, we randomized 22 patients to receive ticlopidine and aspirin (ticlopidine group) ...
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: Alterations of platelet, coagulation, and fibrinolysis markers were investigated to determine the indications for antithrombotic therapy in patients with different clinical categories of acute cerebral infarction. Marked platelet activation was observed in platelet function tests, including measurements of platelet-specific proteins and platelet survival, platelet scintigraphy in the brain, and platelet fibrinogen binding assay, in patients with atherothrombotic stroke. Among patients with ath...
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Jun 15, 1996·Blood23.63
#1Alan D. Michelson (UMMS: University of Massachusetts Medical School)H-Index: 84
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Feb 15, 1996·Journal of Clinical Investigation14.81
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Highly reactive oxygen species rapidly inactivate nitric oxide (NO), and endothelial product which inhibits platelet activation. We studied platelet inhibition by NO in two brothers with a cerebral thrombotic disorder. Both children had hyperreactive platelets, as determined by whole blood platelet aggregometry and flow cytometric analysis of the platelet surface expression of P-selectin. Mixing experiments showed that the patients'platelets behaved normally in control plasma; however, control p...
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Elevated levels of shear stress that occur in stenotic arteries may induce platelet aggregation and initiate thrombosis. Shear-induced platelet aggregation (SIPA) was studied in groups of ischemic stroke patients and normal subjects using a viscometric-flow cytometric technique. Twenty-three patients who sustained an ischemic stroke that was not of cardiac origin were included in this study, and were classified either as atherosclerotic (n = 15) or as lacunar (n = 8) stroke patients. The results...
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Jul 1, 1995·Drugs9.55
#1Karsten Schrör (HHU: University of Düsseldorf)H-Index: 63
Antiplatelet therapy has become a useful means of preventing acute thromboembolic artery occlusions in cardiovascular diseases. The rationale for this is an enhanced activity of circulating platelets and release of platelet-derived vasoactive mediators, probably due to endothelial dysfunction. This review discusses the current status of 4 major classes of antiplatelet compounds : (i) aspirin and related drugs active via cyclo-oxygenase product formation ; (ii) thienopyridines (ticlopidine and cl...
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: Platelet activation plays an important role in the pathomechanisms of arterial vascular disorders including stroke, peripheral arterial disease (PAD), and myocardial infarction. Circulating activated platelets may be useful markers of local thrombotic events occurring in these diseases. Using flow cytometry circulating activated platelets can be detected by determining: 1. the platelets' shape change on the basis of the different light scatter properties of discocytes and spherocytes, 2. the e...
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Ischemic stroke (IS) constitutes a relevant health concern recently in younger population causing permanent cognitive and function-limiting disability and ranks as the 3rd cause of death in Egypt after cardiac and hepatic diseases. Platelet activation has a crucial mechanism in arterial thrombogenesis, thus in pathophysiology of IS. Surface expression of P-selectin (CD62P) reflects platelet activation and measured by flowcytometry. The purpose of the study is to evaluate whether platelet activit...
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Expression of the scavenger receptor CD36 on lymphocytes is intriguing. We observed that a minor subpopulation of lymphocytes expressed CD36 on the cell surface. We investigated the source of CD36 and also the proliferation and cytokine production of these CD36 CD4 lymphocytes. Flow cytometry analysis and immunofluorescence microscopy showed that CD36 platelets were responsible for CD36 detection on lymphocytes. CD36 was then used as a tool to characterize lymphocytes with bound platelets. Activ...
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