Platelets are mitogenic for periosteum-derived cells.

Published on Sep 1, 2003in Journal of Orthopaedic Research2.728
· DOI :10.1016/S0736-0266(03)00053-6
Reinhard Gruber41
Estimated H-index: 41
(University of Vienna),
Florian A. Karreth26
Estimated H-index: 26
+ 2 AuthorsGeorg Watzek49
Estimated H-index: 49
(University of Vienna)
Sources
Abstract
The early stages of bone regeneration are associated with a high mitogenic activity of periosteal cells. Here we addressed the question of whether platelets that accumulate within the developing haematoma can account for this tissue response. Addition of platelets, platelet-released supernatants, platelet membranes, and microparticles to bovine periosteum-derived cells resulted in an increase in 3H-thymidine incorporation; lipid extracts had no effect. Platelet-released supernatants retained their activity after incubation at 56 °C, but not at 100 °C. Gel chromatographic analysis revealed the highest mitogenic activity at approximately 35 kD. Of the factors released from activated platelets, basic fibroblast growth factor (bFGF) and platelet-derived growth factor (PDGF) increased 3H-thymidine incorporation. The mitogenic activity of platelet-released supernatants was decreased by anti-PDGF, and anti-bFGF antibodies. Platelet-released supernatants increased the number of proliferating periosteum-derived cells as determined by the expression pattern of Ki67. Platelet-released supernatants also resulted in a stimulation of cell proliferation in periosteal explants. These results suggest that platelets have the potential to stimulate the mitogenic response of the periosteum during bone repair.
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