Role of Amylase-α2A Autoantibodies in the Diagnosis of Autoimmune Pancreatitis

Published on Oct 1, 2015in Pancreas2.92
· DOI :10.1097/MPA.0000000000000417
María Sánchez Castañón1
Estimated H-index: 1
(University of Verona),
Valeria Zuliani9
Estimated H-index: 9
+ 6 AuthorsLuca Frulloni58
Estimated H-index: 58
Objectives Several serological markers have been reported in autoimmune pancreatitis (AIP) patients. However, only serum IgG4 (sIgG4) is available in clinical practice for AIP diagnosis. Antiamylase α antibodies (AMY-α Abs) have been proposed to diagnose AIP. This study evaluates the utility of AMY-α Abs and sIgG4 for AIP diagnosis. Methods Twenty-five AIP patients, 84 disease control groups (31 chronic pancreatitis, 30 acute pancreatitis, 23 pancreatic adenocarcinoma), and 59 healthy donors were prospectively studied. The AMY-α Abs were determined by homemade enzyme-linked immunosorbent assay and sIgG4 by nephelometry. Results Increased sIgG4 were detected to be present in 52% of AIP, 5% in control groups, and 0% in healthy donors, and AMY-α Abs, respectively, in 76%, 36%, and 2%. sIgG4 was elevated in 92% of the 13 patients with type 1 AIP, but in none of 3 with type 2 and of 8 with not otherwise specified AIP. The AMY-α Abs were present in 79%, 67%, and 75% of type 1, type 2, and not otherwise specified AIP, respectively. Sensitivity and specificity of AMY-α Abs were 76% and 78%, and of sIgG4 50% and 94%. By combining the 2 serological markers, sensitivity was 41%, and specificity was 99%. Conclusions The AMY-α Abs may help to diagnosis of AIP and to differentiate AIP subtypes.
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