Amniotic fluid l-ergothioneine concentrations in pregnant sheep after natural mating and transfer of vitrified/thawed in-vitro produced embryos.

Published on Oct 1, 2015in Research in Veterinary Science1.892
· DOI :10.1016/J.RVSC.2015.09.003
Salvatore Sotgia25
Estimated H-index: 25
(University of Sassari),
Angelo Zinellu31
Estimated H-index: 31
(University of Sassari)
+ 7 AuthorsSergio Ledda27
Estimated H-index: 27
(University of Sassari)
Abstract l -ergothioneine levels were measured in amniotic fluid of pregnant sheep after natural mating and transfer of vitrified/thawed in-vitro produced embryos. Amniotic fluids were collected between 60 and 65 and 80–85 days of gestation and analysed by an ultra-performance liquid chromatographic (UPLC) method with fluorescence detection. l -Ergothioneine concentrations ranged between 0.23 and 9.36 μmol/L and were significantly higher in pregnancy obtained by the transfer of vitrified/thawed in-vitro produced embryos. Conversely, no significant changes in amniotic fluid l -ergothioneine concentrations were observed according to the stages of pregnancy considered in this study. These findings suggest that l -ergothioneine concentrations, are not affected as much by the gestational age, but rather by the method used to induce the pregnancy. On the whole, the measurement of l -ergothioneine in amniotic fluid could serve as a useful biomarker of oxidative stress and/or inflammatory state in pregnancy.
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