Simultaneous quantitative analysis of Listeria monocytogenes and Staphylococcus aureus based on antibiotic-introduced lateral flow immunoassay

Food poisoning caused by microorganisms has caused widespread concern. Herein, a highly sensitive on-site screening test strip for the detection of different pathogenic microorganisms (Listeria monocytogenes and Staphylococcus aureus) was designed. In this analysis platform, colloidal gold-coupled vancomycin was used as a signal unit to label Gram-positive bacteria, and highly sensitive polyclonal antibodies were used as recognition molecules to...