Isolation and Molecular Identification of Avian Pathogenic Escherichia coli in Broiler Chickens Suffering from Colibacillosis in some Governorates in Egypt

Published on Apr 1, 2022in European Journal of Vascular and Endovascular Surgery7.069
· DOI :10.21608/EJVS.2021.93246.1274
Waleed Abu El Hammed1
Estimated H-index: 1
,
Hamdy Soufy6
Estimated H-index: 6
+ 2 AuthorsMohamed I. Dessouky2
Estimated H-index: 2
(Cairo University)
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Abstract
This study aimed to evaluate the most predominant strain of avian pathogenic Escherichia coli (APEC) that causes colibacillosis with respiratory manifestation in broiler chickens. Isolation and serotyping identification for the isolates of E. coli were performed. Prevalence of APEC differentiated by detection of the 8 virulence-associated genes “astA, iss, irp2, papC iucD, tsh, vat, and cva/cvi” using multiplex PCR technique in the most important isolates of E. coli was also investigated. Antibacterial sensitivity test for antibiotics disc and oregano at different concentrations (20%, 15%, 10%, and 5%), were estimated for the most important isolates of E. coli. A total of 50 broiler farms were located in Gharbia, Giza, Menoufia, Bahaira, and Dakahliah Governorates were examined. Specimens from lung, liver, spleen, and kidney from diseased birds were used for bacteriological examination. Results revealed that E. coli O27 was the most predominant serotype strain (11 out of 35 isolates), followed by the serotypes of O146 (4/35), O Untypable serotypes (4/35), and the (1/35) of each serotype of O8, O20, O44, O78, O114, O125, O142, O152, O153, O158, O164, and O166. One isolate of APEC O27 out of 11 isolates has 6 virulence-related genes “astA, iss, papC, iucD, vat, and cva/cvi”. Difloxacin and oregano at a concentration of 20% have the highest percentage (100%) of sensitivity against 11 isolates of E. coli O27 strains. In conclusion, E. coli O27 was the most predominant serotype infecting farms of broiler chickens. Difloxacin and oregano 20% could be used for the treatment of APEC O27.
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#1Lidiane Diniz do Nascimento (UFPA: Federal University of Pará)H-Index: 5
#2Angelo Antônio Barbosa de Moraes (Museu Paraense Emílio Goeldi)H-Index: 2
Last. Lênio José Guerreiro de Faria (UFPA: Federal University of Pará)H-Index: 7
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Spice plants have a great influence on world history. For centuries, different civilizations have used them to condiment the foods of kings and nobles and applied them as embalming preservatives, perfumes, cosmetics, and medicines in different regions of the world. In general, these plants have formed the basis of traditional medicine and some of their derived substances have been utilized to treat different human diseases. Essential oils (EOs) obtained from these plants have been also used as t...
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#1Manita Subedi (Government of Nepal)H-Index: 1
#2Himal LuitelH-Index: 11
Last. Dhiraj Kumar Chaudhary (TU: Tribhuvan University)H-Index: 17
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Background Avian pathogenic Escherichia coli (APEC) are causative agent of extraintestinal infections, collectively known as colibacillosis, which results significant losses in poultry industries. The extraintestinal survival of E. coli is facilitated by numerous virulence factors which are coded by virulence genes. This study was conducted to find out the pattern of antibiotic resistance and virulence genes content in the APEC strains isolated from broiler chickens at National Avian Disease Inv...
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#1Jin-Hyung Lee (Yeungnam University)H-Index: 36
#2Yong-Guy Kim (Yeungnam University)H-Index: 22
Last. Jintae Lee (Yeungnam University)H-Index: 62
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Aims Urinary tract infections are caused primarily by uropathogenic Escherichia coli (UPEC), and indwelling catheters are usually colonized by UPEC biofilms tolerant to common antibiotics. Hence, UPEC biofilms pose a substantial challenge, and there is an urgent need for effective control strategies. Methods and Results In this study, 79 essential oils were screened for antibiofilm ability against UPEC. Components of active oils were identified, and their antibiofilm activities were also investi...
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The global incidence of foodborne infections and antibiotic resistance is recently increased and considered of public health concern. Currently, scarcely information is available on foodborne infections and ESBL associated with poultry and beef meat in Egypt. In total, 180 chicken and beef meat samples as well as internal organs were collected from different districts in northern Egypt. The samples were investigated for the prevalence and antibiotic resistance of Salmonella enterica serovars and...
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Essential oils of oregano are widely recognized for their antimicrobial activity, as well as their antiviral and antifungal properties. Nevertheless, recent investigations have demonstrated that these compounds are also potent antioxidant, anti-inflammatory, antidiabetic and cancer suppressor agents. These properties of oregano essential oils are of potential interest to the food, cosmetic and pharmaceutical industries. The aim of this manuscript is to review the latest evidence regarding essent...
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#1Hercules Sakkas (RMIT: RMIT University)H-Index: 15
#2Chrissanthy Papadopoulou (RMIT: RMIT University)H-Index: 21
: For centuries, plants have been used for a wide variety of purposes, from treating infectious diseases to food preservation and perfume production. Presently, the increasing resistance of microorganisms to currently used antimicrobials in combination with the appearance of emerging diseases requires the urgent development of new, more effective drugs. Plants, due to the large biological and structural diversity of their components, constitute a unique and renewable source for the discovery of ...
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#1Meradi LaaremH-Index: 1
Last. Mohammed Timinouni (Pasteur Institute)H-Index: 20
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Introduction: The emergence and spread of quinolone-resistant Escherichia coli in poultry products puts consumers at risk of exposure to the strains of E. coli that resist antibiotic treatment. The objective of this study was to define the prevalence and virulence potential of poultry-associated nalidixic acid (NAL)-resistant E. coli in the Annaba city, Algeria. Methodology: In total, 33 samples of retail chicken meat were purchased from various butcher shops and examined for bacterial contamina...
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Last. M. I. Corrêa de SáH-Index: 1
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Poultry colibacillosis due to Avian Pathogenic Escherichia coli (APEC) is responsible for several extra-intestinal pathological conditions, leading to serious economic damage in poultry production. The most commonly associated pathologies are airsacculitis, colisepticemia, and cellulitis in broiler chickens, and salpingitis and peritonitis in broiler breeders. In this work a total of 66 strains isolated from dead broiler breeders affected with colibacillosis and 61 strains from healthy broilers ...
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