Study on anti-melanoma effect of deer blood crystal on macrophages

Volume: 36, Issue: 8, Pages: 939 - 943
Published: Jan 1, 2020
Abstract
目的:探索鹿血晶对巨噬细胞杀伤黑色素瘤细胞的影响.方法:细胞增殖试剂盒(CCK8)检测细胞活力,流式细胞术检测细胞凋亡和巨噬细胞吞噬情况.qRT-PCR法检测炎症因子mRNA表达水平,ELISA检测上清炎症因子浓度.蛋白印迹法检测mTOR信号通路下游4EBP和S6磷酸化水平.结果:鹿血晶刺激后Raw264.7细胞培养上清抑制B16F10细胞活力,促进B16F10细胞凋亡.鹿血晶促进Raw264.7细胞吞噬B16F10细胞,增强Raw264.7细胞活力,并上调炎症因子iNOS、TNF-α、IL-6、IL-1β的mRNA表达,促进上述炎症因子分泌,上调4EBP和S6蛋白磷酸化水平.结论:鹿血晶通过活化mTOR信号通路提高巨噬细胞对黑色素瘤细胞的杀伤作用...
Paper Details
Title
Study on anti-melanoma effect of deer blood crystal on macrophages
Published Date
Jan 1, 2020
Volume
36
Issue
8
Pages
939 - 943
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