Determination of Vascular Endothelial Growth Factor (VEGF) in Cell Culture Medium by Gold-Coated Magnetic Nanoparticle Based Label-Free Electrochemical Impedance Spectroscopy (EIS)

A gold-coated magnetic nanoparticle (MNPs@Au) based label-free electrochemical impedance spectroscopy (EIS) sensor is reported for determining vascular endothelial growth factor (VEGF) in cell culture media. VEGF antibodies (Ab) were modified on MNPs@Au to construct an Ab-bound magnetic complex (MNPs@Au-Ab) that was magnetically immobilized on a magnetic glassy carbon electrode (mGCE). The VEGF is captured on the MNPs@Au-Ab/mGCE, leading to...