Transformation of multicolour flow cytometry data with OTflow prevents misleading multivariate analysis results and incorrect immunological conclusions

The rapid evolution of the flow cytometry field, currently allowing the measurement of 30-50 parameters per cell, has led to a marked increase in deep multivariate information. Manual gating is insufficient to extract all this information. Therefore, multivariate analysis (MVA) methods have been developed to extract information and efficiently analyze the high-density multicolour flow cytometry (MFC) data. To aid interpretation, MFC data are...