Cytometry Part A
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#2You-Hong ZhangH-Index: 1
Last. Jose C. Merchuk (BGU: Ben-Gurion University of the Negev)H-Index: 31
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The potential of flow cytometry for the study of changes in prodigiosin on the cell surface of Serratia marcescens is of academic and practical interest. This is because S. marcescens can produce prodigiosin, a secondary metabolite, with potential use as a cancer-cell inhibitor. In this study, three groups of bacterial cultures with different carbon sources were compared, and the effect of the addition of cAMP to the sucrose-based culture was studied. Both cellular morphology and DNA content wer...
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#1Eva M. Temsch (University of Vienna)H-Index: 24
#2Petr Koutecký (Sewanee: The University of the South)H-Index: 10
Last. Jaroslav Doležel (CAS: Academy of Sciences of the Czech Republic)H-Index: 70
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The estimation of nuclear DNA content has been by far the most popular application of flow cytometry in plants. Because flow cytometry measures relative fluorescence intensities of nuclei stained by a DNA fluorochrome, ploidy determination, and estimation of the nuclear DNA content in absolute units both require comparison to a reference standard of known DNA content. This implies that the quality of the results obtained depends on the standard selection and use. Internal standardization, when t...
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#1Francesco Grandoni (Canadian Real Estate Association)H-Index: 4
#2Maria Carmela Scatà (Canadian Real Estate Association)H-Index: 9
Last. Jamal Hussen (King Faisal University)H-Index: 11
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Monocytes are bone marrow derived innate myeloid cells that circulate in the blood and play important roles in infection and inflammation. As part of the mononuclear phagocytic system, monocytes provide innate effector functions, support the adaptive immune response, and play a role in the maintenance of tissue homeostasis. In addition to their role in sensing pathogen-associated molecular patterns using several pattern recognition receptors, monocytes are characterized by their ability to inges...
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#1Tao Peng (BIT: Beijing Institute of Technology)
#2Xinyue Su (BIT: Beijing Institute of Technology)
Last. Qin Li (BIT: Beijing Institute of Technology)
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Despite the wide use of cytometry for white blood cell classification, the performance of traditional cytometers in point-of-care testing remains to be improved. Microfluidic techniques have been shown with considerable potentials in the development of portable devices. Here we present a prototype of microfluidic cytometer which integrates a three-dimensional hydrodynamic focusing system and an on-chip optical system to count and classify white blood cells. By adjusting the flow speed of sheath ...
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#1David W. Galbraith (UA: University of Arizona)H-Index: 89
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#1Wei HuH-Index: 6
#2Xiang SongH-Index: 6
Last. Yong ZhaoH-Index: 6
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#1Takuto Nogimori (Osaka University)
#2Yuko Sugawara (BD: Becton Dickinson)
Last. Takuya YamamotoH-Index: 21
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This 31-parameter panel was developed for simultaneously measuring multiple immune cell populations including T cells, B cells, natural killer cells, dendritic cells, monocytes, and hematopoietic progenitor cells in human peripheral blood mononuclear cells. This panel enables the capture of individual immune dynamics and assessments of single-cell changes in the immune system that are associated with aging and diseases. This panel includes markers to separate the differentiation status of each c...
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#1Attila Tárnok (Leipzig University)H-Index: 11
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#1Fei Peng (Harvard University)H-Index: 1
#2Sinyoung Jeong (Harvard University)H-Index: 14
Last. Conor L. Evans (Harvard University)H-Index: 27
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Neurological disorders affect hundreds of millions of people around the world, are often life-threatening, untreatable, and can result in debilitating symptoms. The high prevalence of these disorders, which feature biochemical or structural abnormalities in neuronal systems, has spurned innovations in both rapid and early detection to assist in the selection of appropriate treatment strategies to improve the patients' quality of life. Plasmonic nanoparticles (PNPs), a versatile and promising cla...
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#1Rita Folcarelli (Radboud University Nijmegen)H-Index: 5
#2Selma van StaverenH-Index: 6
Last. Oscar F. van den BrinkH-Index: 2
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INTRODUCTION The rapid evolution of the flow cytometry field, currently allowing the measurement of 30-50 parameters per cell, has led to a marked increase in deep multivariate information. Manual gating is insufficient to extract all this information. Therefore, multivariate analysis (MVA) methods have been developed to extract information and efficiently analyse the high-density multicolour flow cytometry (MFC) data. To aid interpretation, MFC data are often logarithmically transformed before ...
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Top fields of study
Molecular biology
Cytometry
Flow cytometry
Biology
Cell biology