Morphological switch is associated with increase in cell–cell contacts, ALP , and confluence above a minimum island area to perimeter ratio

During osteogenic differentiation in vitro, stem-like cells seeded at a low-density spread and are isolated. As the cells proliferate and mature, they become more cuboidal in shape with more cell-cell contacts. However, the coordination of this switch in cell morphology from elongated to cuboidal, cell-cell contacts, and differentiation is not known. In this work, we present results from experiments and a simulation of cell proliferation on...