Determination of (R)-(+)- and (S)-(−)-Nicotine Chirality in Puff Bar E-Liquids by ¹H NMR Spectroscopy, Polarimetry, and Gas Chromatography–Mass Spectrometry

Tobacco products generally contain tobacco-derived nicotine (TDN; having ∼99+% (S)-(-)-nicotine). Recent United States regulation has led some producers to transition to synthetic ("tobacco-free") nicotine. For example, Puff Bar is now marketed with tobacco-free nicotine (TFN; presumed to be racemic). To evaluate the claim that these new products contain TFN, we evaluated the presence of the two nicotine optical isomers by 1H NMR spectroscopy,...