Linear and Branched Forms of Short Antimicrobial Peptide-IRK Inhibit Growth of Multi Drug Resistant Staphylococcus aureus Isolates from Mastitic Cow Milk
#1S.K. Gupta(IVRI: Indian Veterinary Research Institute)H-Index: 2
#2Abhishek(IVRI: Indian Veterinary Research Institute)H-Index: 3
Last. Bablu Kumar(IVRI: Indian Veterinary Research Institute)H-Index: 4
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The extensive use of antibiotics in medical, as well as in veterinary practices has led to the development of antibiotic resistance which is of increasing global concern. The so called “superbugs” or the multi-drug resistant bacteria have made the present day antibiotics less effective. Therefore, search of an alternative is the need of hour, and for which antimicrobial peptides (AMPs) could be a good approach. AMPs are relatively short (12 to 100 amino acids), amphipathic peptides isolated from...
The COVID-19 disease is caused by SARS-CoV-2 and spreading rapidly worldwide with extremely high infection rate. Since effective and specific vaccine is not available to combat the deadly COVID-19, the objective of our study was to design a multi-epitope vaccine using immunoinformatics approach with translational implications. Nucleocapsid (N) protein of SARS-CoV-2 is stable, conserved and highly immunogenic along with being less prone to mutations during infection, which makes it a suitable can...
Last. John D. Wade(Florey Institute of Neuroscience and Mental Health)H-Index: 60
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Antimicrobial resistance (AMR) is one of the greatest threats to human health that, by 2050, will lead to more deaths from bacterial infections than cancer. New antimicrobial agents, both broad-spectrum and selective, that do not induce AMR are urgently required. Antimicrobial peptides (AMPs) are a novel class of alternatives that possess potent activity against a wide range of Gram-negative and positive bacteria with little or no capacity to induce AMR. This has stimulated substantial chemical ...
#3Sanjay Singh(IIT: Indian Institute of Technology (BHU) Varanasi)H-Index: 20
The overuse of antibiotics has led to emergence of antimicrobial resistance, and as a result, antibacterial peptides (ABPs) are receiving significant attention as an alternative. Identification of effective ABPs in lab from natural sources is a cost-intensive and time-consuming process. Therefore, there is a need for the development of in silico models, which can identify novel ABPs in protein sequences for chemical synthesis and testing. In this study, we propose a deep learning classifier name...
The current study was conducted to isolate and identify multidrug-resistant Staphylococcus aureus (MDR-SA) from mastitis milk samples and to determine their antimicrobial susceptibility pattern. A total of 48 bovine mastitis (BM) milk samples were collected from different parts of the Rangpur division, Bangladesh. After the collection of milk samples, mastitis was confirmed using the California mastitis test. Isolation and identification of Staphylococcus aureus were performed using conventional...
#1Qiang Ren(Xinjiang Production and Construction Corps)H-Index: 1
#2Guanghua Liao(Xinjiang Production and Construction Corps)H-Index: 1
Last. Wei Chen(Xinjiang Production and Construction Corps)H-Index: 1
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ABSTRACT Staphylococcus aureus is one of the major pathogens causing mastitis in dairy herds. The colonization of dairy cows and subsequent contamination of raw milk by S. aureus, especially strains exhibiting multidrug resistance and biofilm-forming and toxin-producing abilities, remains an important issue for both dairy farmers and public health. In this study, we investigated the prevalence, antimicrobial susceptibility, biofilm formation, and genetic diversity of S. aureus from subclinical b...
BACKGROUND: Infections are one of the leading causes of death worldwide and currently available treatments remain unsatisfactory due to rise in the cases of antimicrobial resistance. Thus, there is a need for the development of new drugs with different mechanisms of action. However, the development of new antimicrobials agents is a long and expensive process. Hence, most of the pharmaceutical companies are looking forward to repurposing the already available drugs against microbial infections. M...
: Antimicrobial peptides in recent years have gained increased interest among scientists, health professionals and the pharmaceutical companies owing to their therapeutic potential. These are low molecular weight proteins with broad range antimicrobial and immuno modulatory activities against infectious bacteria (Gram positive and Gram negative), viruses and fungi. Inability of micro-organisms to develop resistance against most of the antimicrobial peptide has made them as an efficient product w...
In the current investigation, we have compared the potential of statin drugs (Simvastatin, Rosuvastatin and Fluvastatin) as potential anti-bacterial agent by conducting in silico, in vitro and ex vivo studies. In silico study was conducted to check the interaction of statin drugs towards various targets of bacteria. The percentage growth retardation, bacterial growth kinetics, MIC determination, post antibiotic effect and biofilm formation assay were conducted to check the anti-bacterial effect ...
