The Evaluation of Acute Lung Injury Induced by Nitrogen Mustard in Mouse Precision Cut Lung Slices

Nitrogen Mustard (NM; mechlorethamine hydrochloride) is a cytotoxic alkylating agent known to cause progressive pulmonary injury. Its toxic properties elicit extensive tissue remodeling within the respiratory tract, contributing to the development of chronic lung disease. Precision-Cut Lung Slices (PCLS) represent an ex vivo alternative to in vivo studies, minimizing animal numbers while increasing experimental replicates, which is advantageous...