Time-resolved structured illumination microscopy reveals key principles of Xist RNA spreading

Visualizing Xist RNA dynamics The noncoding RNA Xist, which controls the process of X chromosome inactivation in mammals, accumulates and spreads over the chromosome from which it is transcribed. The underlying basis for this unusual behavior is poorly understood. Using a new imaging approach called RNA-SPLIT for time-resolved analysis of Xist RNA molecules at super-resolution, Rodermund et al. analyzed fundamental parameters of Xist RNA...