Original paper
Enhancement of protein thermostability by three consecutive mutations using loop-walking method and machine learning
Abstract
We developed a method to improve protein thermostability, “loop-walking method”. Three consecutive positions in 12 loops of Burkholderia cepacia lipase were subjected to random mutagenesis to make 12 libraries. Screening allowed us to identify L7 as a hot-spot loop having an impact on thermostability, and the P233G/L234E/V235M mutant was found from 214 variants in the L7 library. Although a more excellent mutant might be discovered by screening...
Paper Details
Title
Enhancement of protein thermostability by three consecutive mutations using loop-walking method and machine learning
Published Date
Jun 4, 2021
Journal
Volume
11
Issue
1
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Notes
History