Nonenzymatic Electrochemical Sensor with Ratiometric Signal Output for Selective Determination of Superoxide Anion in Rat Brain

There is still an urgent need to develop reliable analytical methods of O2•– in vivo for deeply elucidating the roles of O2•– playing in the brain. Herein, a nonenzymatic electrochemical sensor with ratiometric signal output was developed for an in vivo analysis of O2•– in the rat brain. Diphenylphosphonate-2-naphthol ester (ND) was designed and synthesized as a specific recognition molecule for the selective determination of O2•–. An anodic...