Covalent and Ionic Bonding between Tannin and Collagen in Leather making and shrinking: A MALDI-ToF study

Published on Feb 25, 2021in Journal of Renewable Materials1.341
· DOI :10.32604/JRM.2021.015663
Antonio Pizzi79
Estimated H-index: 79
Collagen powder hydrolysates were reacted with a solution of commercial mimosa bark tannin extract. The mix- ture was prepared at ambient temperature and prepared at 80°C to determine what reactions, if any, did occur between the collagen protein through its amino acids and the polyphenolic condensed tannin. The reaction pro- ducts obtained were analyzed by matrix assisted laser desorption ionization time-of-flight (MALDI ToF) mass spectrometry. Reactions between the two materials did appear to occur, with the formation of a relatively small proportion of covalent and ionic linkages at ambient temperature but a considerable proportion of covalent lin- kages tannin-protein amino acids and the disappearance of ionic bonds. The linkages between the two materials appeared to be by amination of the phenolic –OHs of the tannin by the amino groups of the non-skeletal side chains of arginine, and by esterification by the –COOH groups of glutamic and aspartic acid of the aliphatic alcohol-OH on the C3 site of the flavonoid units heterocycle of the tannin. The proportion of covalent linkages increases markedly and predominate with increasing temperatures. This tightening of the tannin-protein covalent network formed may be an additional contributing factor both to leather wear resistance and performance as well to leather shrinking when this is subjected to excessive temperatures.
📖 Papers frequently viewed together
18 Citations
12 Citations
42 Citations
#1Achille Gnassiri Wedaïna (University of Ngaoundéré)H-Index: 1
#2Antonio PizziH-Index: 79
Last. Raphaël KuenyH-Index: 3
view all 8 authors...
The Piptadeniastrum Africanum bark tannin extract was characterized using MALDI TOF, ATR-FT MIR. It was used in the development of a resin with Vachellia nilotica extract as a biohardener. This tannin is consisting of Catechin, Quercetin, Chalcone, Gallocatechin, Epigallocatechin gallate, Epicatechin gallate. The gel time of the resin at natural pH (pH = 5.4) is 660 s and its MOE obtained by thermomechanical analysis is 3909 MPa. The tenacity of Urena lobata fibers were tested, woven into unidir...
3 CitationsSource
#1Saman Ghahri (TMU: Tarbiat Modares University)H-Index: 5
#2Antonio Pizzi (University of Lorraine)H-Index: 79
Two different types of commercial tannins, namely chestnut (a hydrolysable tannin) and mimosa (a condensed flavonoid tannin), were used with two different types of hardener, namely hexamine and glyoxal, to upgrade soy-based adhesives for bonding of wood particleboard. Different soy-based adhesive formulations were tested by differential scanning calorimetry (DSC) and by thermomechanical analysis (TMA). The physical properties of different adhesive formulations such as viscosity, solid contents, ...
49 CitationsSource
#1F.J. Santiago-Medina (University of Lorraine)H-Index: 9
#2Antonio PizziH-Index: 79
Last. Alain CelzardH-Index: 64
view all 5 authors...
Reaction of a condensed flavonoid tannin, namely mimosa tannin extract with a hexamethylene diamine, has been investigated. For that purpose, catechin was also used as a flavonoid model compound and treated in similar conditions. Solid-state cross-polarisation/magic-angle spinning (CP-MAS) carbon 13 nuclear magnetic resonance (13C NMR) and matrix assisted laser desorption ionisation time of flight (MALDI-ToF) mass spectroscopy studies revealed that polycondensation compounds leading to resins we...
15 CitationsSource
25 CitationsSource
#1Flavia Lega Braghiroli (University of Lorraine)H-Index: 13
#2Vanessa Fierro (University of Lorraine)H-Index: 60
Last. Alain Celzard (University of Lorraine)H-Index: 64
view all 8 authors...
Abstract Reaction of condensed (flavonoid) mimosa tannin extracts with concentrated aqueous ammonia has been investigated. For that purpose, catechin was also used as a flavonoid model compound and treated in similar conditions. Solid state CP-MAS 13 C NMR and MALDI-ToF spectroscopy studies revealed that, unlike what was recently theorised, amination is not always regioselective and leading to the conversion of one single OH group in C4′ into NH 2 . In the present study, new reactions have been ...
45 CitationsSource
#1Wentao Liu (Sichuan University)H-Index: 12
#2Guoying Li (Sichuan University)H-Index: 21
Abstract To further understand the kinetics of the thermal denaturation of type I collagen, the 16 and 8 mg/mL bovine hide collagen solutions were studied by differential scanning calorimetry at different heating rates. The activation energy of the denaturation process was examined utilizing the differential (Friedman) and the integral (Ozawa-Flynn-Wall) isoconversional methods, and several kinetic models were evaluated using the method of multivariate non-linear regression. Besides, the denatur...
33 CitationsSource
#1Antonio PizziH-Index: 79
#2Harald Pasch (Stellenbosch University)H-Index: 53
Last. Samuele GiovandoH-Index: 10
view all 4 authors...
The structures of six commercial hydrolyzable tannins, chestnut, oak, tara, sumach, chinese gall, and turkey gall tannins have been examined by matrix-assisted laser desorption/ionization-time-of-flight (MALDI-TOF) mass spectrometry. Their oligomeric structures and structure distributions have been defined. Degradation products of rather different structure than what previously reported were present. Different galloyl glucose monomers were observed for chestnut and oak tannin extracts and in chi...
56 CitationsSource
#1Koh HashidaH-Index: 10
#2Rei MakinoH-Index: 8
Last. Seiji OharaH-Index: 15
view all 3 authors...
22 CitationsSource
Differential scanning calorimetry (DSC) is a very useful tool to research the hydrothermal stability of collagen which has also been defined in the leather industry as denaturation temperature (Td). It has been widely used to describe the tanning power of materials, according to changes of Td. In this paper, changes in hydrothermal stability of collagen with several catechin-metal compounds were described using DSC curves. The Td results lead us to conclude that the hydrothermal stability of col...
4 Citations
The rather novel picture of chestnut wood tannin, in situ in the wood, which emerges from matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF-MS) is that of a pervasive and extended random tridimensional macromolecular network, formed by pentagalloylglucose clusters linked to each other, to form chains and encrust the wood constituents matrix. Up to pentagalloylglucose trimers were identified in the MALDI-TOF analysis of the commercial chestnut tannin extract,...
69 CitationsSource
Cited By1