Microplate-based antithrombin activity bioassay for Shuxuetong Injection through aptamer-thrombin capturing coupled with chromogenic substrate hydrolysis

Shuxuetong Injection (SXT) is one kind of herbal injections derived from leech (Whitmania pigra) and earthworm (Pheretima aspergillum). It exhibits powerful therapeutic effects on “blood stasis and stagnation (yu xue yu zhi)”, particularly in patients with stroke and myocardial infarction. However, adverse reactions caused by quality problems occur occasionally. We described an aptamer-based bioassay for antithrombin activity of SXT. The...