Production and characterization of polyhydroxyalkanoates from industrial waste using soil bacterial isolates.

Published on Feb 15, 2021in Brazilian Journal of Microbiology2.476
· DOI :10.1007/S42770-021-00452-Z
Shreya Shah1
Estimated H-index: 1
,
Anil Kumar121
Estimated H-index: 121
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Abstract
Nowadays when conventional plastic is being looked as a menace, the possibility of it being replaced with polyhydroxyalkanoates (PHAs) which are biodegradable, environment friendly and biocompatible thermoplastics is not remote. PHAs are a fascinating group of biopolyesters stored within the cytoplasm of numerous bacterial cells as energy and carbon reserves. PHAs signify the best promising biological substitute to certain conventional petrochemical plastics which have wide range of applications in different industries such as biomedical sector, packaging, toners for printing, and adhesives for coating, etc. In the present study, PHAs producing bacterial strains were screened by Sudan black B staining and confirmed by Nile blue A staining. Out of forty bacterial strains showing positive results, six bacterial strains exhibited comparatively higher PHAs production. The highest PHAs producing bacterial strain was identified using 16s rRNA sequencing. Optimization of process parameters was performed by using one factor at a time (OFAT) approach. The isolated bacterium was able to synthesize PHAs when various agro-industrial wastes such as domestic kitchen waste, mixed fruit pulp, sugarcane molasses, and waste flour from bread factory were screened as a carbon substrate in the growth medium. The results showed accumulation of 44.5% PHAs of cell dry weight using domestic kitchen waste as carbon substrate. The characterization of biopolymers was performed using FTIR and XRD analysis. The commercial exploitation of results of this study may serve twin purposes of addressing the challenge of high production cost of PHAs being the major constraint in replacing petro-based plastics as well as address the problem of disposal of recurring domestic kitchen waste and other agro-industrial waste.
References49
Newest
Abstract In view of risk coupled with synthetic polymer waste, there is an imperative need to explore biodegradable polymer. On account of that, six PHAs producing bacteria were isolated from mangrove forest and affilated to the genera Bacillus & Pseudomonas from morpho-physiological characterizations. Among which the potent PHAs producer was identified as Bacillus megaterium OUAT 016 by 16S rDNA sequencing and in-silico analysis. This research addressed a comparative account on PHAs production ...
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#1A. Surendran (Universiti Sains Malaysia)H-Index: 5
#2Manoj Lakshmanan (Universiti Sains Malaysia)H-Index: 6
Last. Kumar Sudesh (Universiti Sains Malaysia)H-Index: 43
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Polyhydroxyalkanoates (PHA) are a potential next generation material to replace some of the petrochemical based plastics. PHAs are polyesters synthesized and stored by various prokaryotes and archaea as water-insoluble inclusions in their cytoplasm. PHAs are usually produced when the microbes are cultured in nutrient limiting conditions with excess carbon sources. Hence this concept is implemented in industries to reduce cost of PHAs produced commercially. Industrially, these biodegradable polye...
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#1Swayamsidha PatiH-Index: 1
#2Sudipta Maity (University of Rajasthan)H-Index: 3
Last. D.P. Samantaray (Yahoo!)H-Index: 4
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Catastrophic global accumulation of non-biodegradable plastic has led to efforts for production of alternative eco-friendly biopolymer. Here, we attempted to produce a biodegradable, cytocompatible and eco-friendly polyhydroxy-butyrate (PHB) from a pigmented Bacillus sp. C1 (2013) (KF626477) through submerged (SmF) and solid-state fermentation (SSF). Under SmF and SSF, 0.60 g l−1 and 1.56 g l−1 of PHB with 0.497 g l−1 of yellow fluorescent pigment (YFP) was produced. Fourier transform infrared (...
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#1Ruby Dwivedi (King George's Medical University)H-Index: 5
#2Rahul Pandey (King George's Medical University)H-Index: 5
Last. Divya Mehrotra (King George's Medical University)H-Index: 15
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ABSTRACT Polyhydroxyalkanoates (PHA) are prokaryotic macromolecules accumulated within the cytoplasm as granules. Due to their suitable mechanical properties, biocompatibility, degradation time, ability to be blended, surface modified, and form copolymers, it is widely used in medical devices and as scaffolds in bone tissue engineering. This review describes in brief the production and extraction sources, physico- chemical characteristic, mechanical properties, degradation rate and applications ...
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#1Guo-Qiang ChenH-Index: 1
#2Xin-Yu Chen (THU: Tsinghua University)H-Index: 2
Last. Jin-Chun Chen (THU: Tsinghua University)H-Index: 34
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Abstract Polyhydroxyalkanoates (PHA) have been produced by several bacteria as bioplastics in industrial scales. PHA commercialization has been challenging during to its complexity and the associated high cost together with instabilities on molecular weights (Mw) and structures, thus instability on thermo- and mechanical properties. PHA high production cost is related to complicated bioprocessing associated with sterilization, low conversion of carbon substrates to PHA products, and poor growth ...
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Abstract Polyhydroxyalkanoates are a fascinating group of biological polymers, which have attracted extensive attention. An effectual PHB accumulating Bacillus cereus strain VIT-SSR1 was isolated from industrial effluent contaminated site. Plackett-Burman design was used to screen the critical factors affecting PHA production. Response surface method was employed to evaluate the interactive effects between the independent variables. The Central composite design was performed to optimize the conc...
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#1Chad Nielsen (USU: Utah State University)H-Index: 2
#2Asif Rahman (UNM: University of New Mexico)H-Index: 10
Last. Charles D. Miller (USU: Utah State University)H-Index: 30
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Summary Polyhydroxyalkanoates (PHAs) are biopolymers with desirable material properties similar to petrochemically derived plastics. PHAs are naturally produced by a wide range of microorganisms as a carbon storage mechanism and can accumulate to significantly high levels. PHAs are an environmentally friendly alternative to their petroleum counterparts because they can be easily degraded, potentially reducing the burden on municipal waste systems. Nevertheless, widespread use of PHAs is not curr...
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#1S. Mohapatra (Orissa University of Agriculture and Technology)H-Index: 1
#2P. R. Mohanta (Orissa University of Agriculture and Technology)H-Index: 1
Last. D.P. Samantaray (Orissa University of Agriculture and Technology)H-Index: 9
view all 6 authors...
Municipal sewage triggers a stress prone environment to accumulate polyhydroxyalkanoates (PHAs) in the cytosol of bacteria. In view of that, different Bacillus species were isolated from municipal sewage and screened for evaluating their efficacy of PHA production. Growth parameters such as temperature, pH, glucose concentration and carbon nitrogen combinations were optimized with respect to higher biomass production as it is analogous to PHA accumulation. Under optimized conditions, the Bacillu...
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#1Anteneh GetachewH-Index: 1
#2Fantahun Woldesenbet (Arba Minch University)H-Index: 1
Polyhydroxybutyrates (PHBs) are macromolecules synthesized by bacteria. They are inclusion bodies accumulated as reserve materials when the bacteria grow under different stress conditions. Because of their fast degradability under natural environmental conditions, PHBs are selected as alternatives for production of biodegradable plastics. The aim of this work was to isolate potential PHB producing bacteria, evaluate PHB production using agro-residues as carbon sources. Among fifty bacterial stra...
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#3A. W. Zularisam (Universiti Malaysia Pahang)H-Index: 14
The rapid industrial and economic development runs on fossil fuel and other energy sources. Limited oil reserves, environmental issues, and high transportation costs lead towards carbon unbiased renewable and sustainable fuel. Compared to other carbon-based fuels, biodiesel is attracted worldwide as a biofuel for the reduction of global dependence on fossil fuels and the greenhouse effect. During biodiesel production, approximately 10% of glycerol is formed in the transesterification process in ...
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