Abstract In this research, a comprehensive and innovative method was established for the qualitative and quantitative analysis of the main components in Mahonia fortune (MF). On the one hand, comprehensive insight of the constituents in MF extracts was achieved with a Q‑Exactive HF Mass Spectrometer using data-independent acquisition method. The identification of 17 compounds was based on comparison with authentic reference standards and the deduction of 119 additional compounds both in positive and negative modes was using the MS-dial strategy and comparison with literature data. The proportion of alkaloids and phenols were the most in MF. On the other hand, an ultra-performance liquid chromatographic-electrospray ionization tandem mass spectrometry (UPLC–ESI-MS/MS) method for the quantification of 25 components in MF extract were developed and validated. The method established provided satisfactory precision and accuracy; acceptable recovery and stability; a good linearity and a reasonable limit of detection. The MF samples from 11 different sources were detected, and relative principal component analysis were applied to discriminate these samples. The variations of Columbamine, Jatrorrhizine, Palmatine and Berberine were suggested as important indicators of MF quality. This study supplies a novel and comprehensive method for the quality evaluation of MF. This research presents a MS based analytical strategy which shows an application potential in the analysis of the chemical constituents in Traditional Chinese Medicine (TCM).
Last. Jianguo Zeng(HAU: Hunan Agricultural University)H-Index: 12
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Isoquinoline alkaloids, which are one of the most important types of alkaloids, are extensively distributed in herbal medicines. However, systematic and comprehensive investigations of the fragmentation behaviours of isoquinoline alkaloids have rarely been reported. Therefore, the goal of the present study is to simultaneously investigate the collision-induced dissociation patterns and the corresponding mechanism of isoquinoline alkaloids by mass spectrometry (MS) combined with computations. Nin...
Abstract Untargeted metabolomic profiling has been widely used in recent years. However, the low reproducibility of the data-dependent acquisition (DDA) strategy presents a major bottleneck that considerably limits the reliability of metabolomic studies in biological and clinical research. The data-independent acquisition (DIA) strategy is proposed to solve the above-mentioned problem, and it is gaining popularity. This paper presents a novel approach for performing metabolomic analysis using an...
In this work, the preparative separation of quaternary ammonium alkaloids from Caulis Mahoniae by pH-zone-refining counter-current chromatography (PZRCCC) was compared to a conventional high-speed counter-current chromatography (HSCCC). A flow-rate changing strategy was performed in conventional CCC separation with two-phase solvent systems with a solution composed of chloroform–methanol–0.5 mM HCl and water (4 : 1.5 : 2, v/v). Compared to conventional CCC separation, the separation of 3.0 g cru...
Since 2012, missing proteins (MPs) investigation has been one of the critical missions of Chromosome-Centric Human Proteome Project (C-HPP) through various biochemical strategies. On the basis of our previous testis MPs study, faster scanning and higher resolution mass-spectrometry-based proteomics might be conducive to MPs exploration, especially for low-abundance proteins. In this study, Q-Exactive HF (HF) was used to survey proteins from the same testis tissues separated by two separating met...
Abstract Ethnopharmacological relevance Mahonia bealei has a long history of medical use in traditional Chinese medicine for the treatment of inflammatory-associated diseases. Despite numerous phytochemical and pharmacological studies, there is a lack of systematic studies to understand the cellular and molecular mechanisms of the anti-inflammatory activity of this plant. Aim of study This study aimed to evaluate the anti-inflammatory activity of the dichloromethane fraction from M. bealei leave...
#1Na Zhang(Beijing University of Chinese Medicine)H-Index: 8
#2Yuelin Song(Beijing University of Chinese Medicine)H-Index: 23
Last. Pengfei Tu(Beijing University of Chinese Medicine)H-Index: 23
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Dual roles have been widely disclosed for Aconiti Lateralis Radix Praeparata (Chinese name: Fuzi) by acting as an effective herbal medicine accompanied with high toxicity risk and narrow therapeutic range. Aconite alkaloids, also known as diterpenoid alkaloids (DAs), have been proved to be primary therapeutic as well as toxic material basis of Fuzi. In the present study, we aim to characterize qualitative profile along with quantitative features of DAs in Fuzi, which could benefit the quality co...
#1Wei‑Kun Ma(CPU: China Pharmaceutical University)H-Index: 1
#2Hui Li(CPU: China Pharmaceutical University)H-Index: 7
Last. Chun-Su Yuan(U of C: University of Chicago)H-Index: 81
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Mahonia bealei is a Chinese folk medicine used to treat various ailments, in particular gastrointestinal inflammation-related illnesses, and palmatine is one of its active constituents. In this study, ApcMin/+ mice, a genetically engineered model, were used to investigate the effects of palmatine on the initiation and progression of gut inflammation and tumorigenesis enhanced by a high-fat diet. The in vitro antiproliferation and anti-inflammation effects of palmatine were evaluated on HT-29 and...
#1Hui Ouyang(JUTCM: Jiangxi University of Traditional Chinese Medicine)H-Index: 12
#2Tianer Li(JUTCM: Jiangxi University of Traditional Chinese Medicine)H-Index: 1
Last. Shilin Yang(JUTCM: Jiangxi University of Traditional Chinese Medicine)H-Index: 11
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In this study, a systematic method was established for the qualitative and quantitative analysis of the major constituents in Flos Scabiosae (FS). First, Ultra-high performance liquid chromatography tandem quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) was developed for the identification of the multi-constituents in FS. A total of 48 compounds (9 phenolic acids, 24 flavonoids, 8 iridoids and 7 others) were unambiguously or tentatively identified, including 25 compounds (flavonoids...
Abstract Lactobacillus mucosae is a natural resident of the gastrointestinal tract of humans and animals and a potential probiotic bacterium. Tounderstand the global protein expression profile and metabolic features of L. mucosae LM1 in the early stationary phase, the QExac-tive™ Hybrid Quadrupole-Orbitrap Mass Spectrometer was used. Characterization of the intracellular proteome identified 842 prot eins,accounting for approximately 35% of the 2,404 protein- coding sequences in the complete geno...
Last. Yong Jiang(PKU: Peking University)H-Index: 31
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In order to solve the bottleneck of reference standards shortage for comprehensive quality control of traditional Chinese medicines (TCMs), a series of strategies, including one single reference standard to determine multi-compounds (SSDMC), quantitative analysis by standardized reference extract (QASRE), and quantitative nuclear magnetic resonance spectroscopy (qNMR) were proposed, and Mahoniae Caulis was selected as an example to develop and validate these methods for simultaneous determinatio...
#1Hui-Peng Song(LNUTCM: Liaoning University of Traditional Chinese Medicine)
#2Hui Zhang(LNUTCM: Liaoning University of Traditional Chinese Medicine)H-Index: 2
Last. Zixuan Ding(LNUTCM: Liaoning University of Traditional Chinese Medicine)
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Abstract null null Lead compound is an important concept for modern drug discovery. In this study, a new concept of lead chemome and an efficient strategy to discover lead chemome were proposed. Compared with the concept of lead compound, lead chemome can provide not only the starting point for drug development, but also the direction for structure optimization. Two traditional Chinese medicines of Mahonia bealei and Mahonia fortunei were used as examples to illustrate the strategy. Based on nat...
The roots of Fissistigma oldhamii (FO) are widely used as medicine with the effect of dispelling wind and dampness, promoting blood circulation and relieving pains, and its fruits are considered delicious. However, Hakka people always utilize its above-ground parts as a famous folk medicine, Xiangteng, with significant differences from literatures. Studies of chemical composition showed there were multiple aristolactams that possessed high nephrotoxicity, pending evaluation research about their ...
