Engineering of an oleate hydratase for efficient C10-Functionalization of oleic acid

Oleate hydratase catalyzes the hydration of unsaturated fatty acids, giving access to C10-functionalization of oleic acid. The resultant 10-hydroxystearic acid is a key material for the synthesis of many biomass-derived value-added products. Herein, we report the engineering of an oleate hydratase from Paracoccus aminophilus (PaOH) with significantly improved catalytic efficiency (from 33 s−1 mM−1 to 119 s−1 mM−1), as well as 3.4 times increased...