Original paper
Endonuclease increases efficiency of osteoblast isolation from murine calvariae
Abstract
Bone is a highly dynamic organ that undergoes remodeling equally regulated by osteoblast-mediated bone formation and osteoclast-mediated bone resorption. To clarify the regulation of osteoblastogenesis, primary murine osteoblasts are required for an in vitro study. Primary osteoblasts are isolated from neonatal calvariae through digestion with collagenase. However, the number of cells collected from one pup is not sufficient for further in vitro...
Paper Details
Title
Endonuclease increases efficiency of osteoblast isolation from murine calvariae
Published Date
Apr 19, 2021
Journal
Volume
11
Issue
1
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Notes
History