A sulfated polysaccharide extracted from Gracilaria lemaneiformis (GLP) with a prominent effect in regulating lipid metabolism was isolated. The molecular weight was 31.5 kDa and it was composed mainly of galactose, glucose and xylose. Fourier-transform infrared (FT-IR) spectrum and nuclear magnetic resonance (NMR) analysis suggested that GLP was composed of the following repeating unit: [3-β-Gal-4(OSO3)-1→4-α-3,6-anhydrogal-2(OSO3)-1→]. GLP could significantly decrease serum total cholesterol, triglyceride and free fatty acid levels and lower alanine aminotransferase and aspartate aminotransferase activities in high-fat-diet mice. Additionally, GLP could keep the body weight and attenuate accumulation of fat surrounding the liver and epididymis induced by high-fat diet. Results of RT-PCR indicated that GLP might regulate lipid metabolism and accelerate free fatty acid oxidation by up-regulating the expression of the PPARα, ACS and CPT1a gene. The present study suggests that GLP may be potentially useful for regulating lipid metabolism.
#1Rui-Bo Jia(SCUT: South China University of Technology)H-Index: 3
#2Juan Wu(SCUT: South China University of Technology)H-Index: 3
Last. Mouming Zhao(SCUT: South China University of Technology)H-Index: 50
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Abstract Three algal polysaccharides (APs) were acquired from Scagassum (SCP), Sargassum fusiforme(Harv.) Setch. (SFP) and Macrocystis pyrifera(L.)Ag. (MAP) by hot water extraction, and their structural characterization and antidiabetic activity were investigated in high fat diet and streptozotocin-induced type 2 diabetic rats. The results revealed that there were obvious differences in extraction yield, molecular weight, compositions of monosaccharide and organic element, molecular morphology, ...
#1Dongying Yang(NUS: National University of Singapore)H-Index: 5
#2Shuai Gao(NUS: National University of Singapore)H-Index: 1
Last. Hongshun Yang(NUS: National University of Singapore)H-Index: 46
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Abstract Sucrose addition contributes to the rheology of carrageenan, which improves the quality of food that includes carrageenan as an additive. In the present study, the rheological behaviours of sucrose-supplemented iota carrageenan at different concentrations (0–20%, w/v) were determined. Easier gelation with a higher gelling temperature (51.4–57.4 °C) could be achieved by sucrose addition, and the trend could be predicted using mathematical models. Application of the Kirkwood-Buff theory d...
#1Lifen Zhang(Henan University of Technology)H-Index: 18
#2Pei Wang(Henan University of Technology)H-Index: 2
Last. Hongshun Yang(NUS: National University of Singapore)H-Index: 46
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Abstract Calcium chloride (1% w/w, CaCl2) and pectin methylesterase (PME) (15 U/mL) were vacuum impregnated (VI) into jujubes to preserve their quality. The nanostructure of jujube pectin was investigated using atomic force microscopy (AFM) to determine the degradation mechanism of pectin. CaCl2 with PME under VI treatment (VI + Ca + PME) maintained jujubes’ quality. Weight loss in VI + Ca + PME group at day 56 was only 60.36% of that in control group (CK). Firmness, soluble solids content, and ...
Last. Dong Liu(SZPT: Shenzhen Polytechnic)H-Index: 6
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This study aimed to evaluate the regulation of lipid metabolism and mechanism of a sulfated polysaccharide from Gracilaria Lemaneiformis (GLP). The results showed that GLP significantly decreased the hepatic total cholesterol (TC) and triglyceride (TG) levels in the serum of high-fat diet mice and prevented body weight gain and alleviated the accumulation of fat. Furthermore, GLP may accelerate the conversion of cholesterol to bile acids by promoting the expression of LxRα and CYP7A1 genes and r...
Last. Chuan-Fang Li(Jining Medical University)H-Index: 1
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Abstract Background Accelerated atherosclerosis in patients suffering from diabetes represents a major cause of morbidity and mortality. The aim of present study was to investigate the protective effects conferred by atorvastatin (AVT) meditated by the HMGCR gene in diabetic rats with atherosclerosis. Methods Serum triglyceride (TG), total cholesterol (TC), high-density lipoprotein cholesterol (HDL-C), low-density lipoprotein cholesterol (LDL-C), very-low-density lipoprotein cholesterol (VLDL-C)...
Last. Chunqing Ai(Dalian Polytechnic University)H-Index: 11
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Abstract Some polysaccharides from Gracilaria lemaneiformis (GLPs) are shown to have various activities, but the effects of GLPs on diet-induced obesity and the underlying mechanisms are unclear. This study indicated that dietary supplementation with GLPs reduced weight gain and improved body composition in high fat diet (HFD)-fed mice without reduced food consumption. Fat accumulation in the liver and adipose tissues was reduced by GLPs, and the damages of HFD on the intestine were repaired. Th...
In the past 2 decades, an increasing prevalence of risk factors for cardiovascular disease, such as obesity, physical inactivity, and diabetes mellitus, has been observed among young adults (aged 18–45 years) living in developed countries. In their Review, Andersson and Vasan discuss the evolving risk factor burden and available epidemiological data on cardiovascular disease in young adults.
Abstract Statins, the most widely used drugs in the Western world, have become a pivotal component in the primary and secondary prevention of vascular diseases. Although benefits have been well documented in younger-than-75-year-old individuals, the value of statins in people aged > 75 years and over is controversial. The CTT meta-analysis calculated an absolute risk reduction of 0.6%/year per 38.7 mg/dl reduction in LDL-C levels in patients aged > 75 years, that would translate into a number ne...
Last. Zhonghua Liu(HAU: Hunan Agricultural University)H-Index: 19
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An increasing amount of evidence suggests that the gut microbiota composition and structure contribute to the pathophysiology of metabolic syndrome (MS), which has been put forward as a new target in the treatment of diet-induced MS. In this work, we aimed to investigate effects of Fuzhuan brick tea polysaccharides (FBTPS) on MS and gut microbiota dysbiosis in high-fat diet (HFD) fed mice and to further investigate whether its attenuation of MS is related to the modulation of gut microbiota. The...
#1Guiqing Zheng(SCUT: South China University of Technology)H-Index: 4
#2Jie Deng(SCUT: South China University of Technology)H-Index: 1
Last. Lin Zhou(Guangdong Pharmaceutical University)H-Index: 7
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Abstract In order to evaluate the release of phenolics and the changes in antioxidant activities, fruits of Crataegus pinnatifida were subjected to in vitro digestion including simulated oral, gastric, small/large intestine digestion. Results showed that totally 37.41 ± 3.09 and 31.51 ± 2.35 mg GAE/g fruit, DW of phenolics were released for Shanlihong and Dajinxing, respectively, and the phenolics released were mainly flavonoids. Catechin, chlorogenic acid, procyanidine B 2 , epicatechin, paraco...
Abstract The study aimed to study the effects on structural characteristics and anti-inflammatory activities of algal sulfated polysaccharides isolated from Gracilaria lemaneiformis (GLP) after a combined treatment of UV irradiation (average irradiance of 6500 mJ/cm2) and H2O2 (50 mmol/L) for various time periods up to 60 minutes. After a 30-minute treatment, the molecular weight and particle size of GLP was decreased by 15 and 2.6 fold, respectively with small but significant decrease in the co...
Gracilariopsis lemaneiformis polysaccharides (GLP) were degraded using pectinase, glucoamylase, cellulase, xylanase, and β-dextranase into low-molecular-weight polysaccharides, namely, GPP, GGP, GCP, GXP, and GDP, respectively, and their antioxidant capacities were investigated. The degraded GLPs showed higher antioxidant activities than natural GLP, and GDP exhibited the highest antioxidant activity. After the optimization of degradation conditions through single-factor and orthogonal optimizat...
Abstract The sulfated polysaccharides extracted from Gracilaria chouae using critic acid extraction and water extraction, respectively, and their digestion and fermentation characteristics were compared in vitro and in vivo. The molecular weight of water extracted polysaccharide of G. chouae (WGCP) was 1.73×103 kDa while critic acid extracted polysaccharide (CGCP) was 31.5 kDa. During stimulated gastrointestinal digestion in vitro, WGCP and CGCP were lightly degraded. However, the glycemic index...