Enantioseparation of 2‐(4‐chlorophenyl)succinic acid by countercurrent chromatography and investigation of injection volume on resolution

2‐(4‐Chlorophenyl)succinic acid was successfully enantioseparated by countercurrent chromatography using hydroxypropyl‐β‐cyclodextrin as chiral selector. A two‐phase solvent system composed of n ‐hexane‐ethyl acetate‐0.1 mol/L phosphate buffer with pH 2.65 (5:5:10, v/v) was selected. Enantioselective liquid–liquid extraction was used to optimize the enantioseparation conditions. Meanwhile, the influence of injection volume on resolution in...