Piwi-interacting RNA

Plasmid

Biology

Guide RNA

Gene

Transposition (logic)

CRISPR interference

Cas9

Mobile genetic elements

Genome

RNA interference

Artificial intelligence

Computer science

Computational biology

Trans-activating crRNA

CRISPR

Transposable element

Genetics

Cell

Transposons have co-opted CRISPR-Cas systems for RNA-guided transposition, using mechanisms that allow target site choice and prevent guide RNA use by canonical CRISPR-Cas systems—features that could be useful for biotechnology applications.

Guide RNA Categorization Enables Target Site Choice in Tn7-CRISPR-Cas Transposons