A Ganoderma atrum polysaccharide alleviated DSS-induced ulcerative colitis by protecting the apoptosis/autophagy-regulated physical barrier and the DC-related immune barrier.

Published on Dec 17, 2020in Food & Function4.171
· DOI :10.1039/D0FO02260H
Bing Zheng3
Estimated H-index: 3
Mengxi Ying3
Estimated H-index: 3
+ 6 AuthorsQiang Yu21
Estimated H-index: 21
Polysaccharides are one of the main active substances in Ganoderma atrum (G. atrum). The purpose of this study was to explore the protective effect of a G. atrum polysaccharide (PSG-1) on DSS-induced colitis and the underlying mechanism. The results showed that PSG-1 could maintain the integrity of the intestinal structure by promoting the expression of goblet cells and levels of tight junction proteins in the colon of DSS-induced colitis mice. Furthermore, PSG-1 relieved the inhibition of Bcl-2 and the overexpression of caspase-3 and caspase-9 caused by DSS. Simultaneously, PSG-1 restored the expression of Atg5, Atg7 and beclin-1 and inhibited the p-akt and p-mTOR levels, suggesting that PSG-1 promoted autophagy via the Akt/mTOR pathway. Moreover, PSG-1 inhibited the content of DCs in the colon and modulated the expression of IL-10 in DCs. In conclusion, PSG-1 alleviated DSS-induced ulcerative colitis by protecting the apoptosis/autophagy-regulated physical barrier and the DC-related immune barrier.
📖 Papers frequently viewed together
12 Authors (Wei Liu, ..., Zongping Liu)
29 Citations
118 Citations
6 Authors (Donglei Liu, ..., Song Zhao)
16 Citations
#1Christian Hartmann (WWU: University of Münster)H-Index: 3
#2Ysabel Alessa Schwietzer (WWU: University of Münster)H-Index: 2
Last. Klaus Ebnet (WWU: University of Münster)H-Index: 32
view all 5 authors...
Abstract Junctional adhesion molecules (JAMs) comprise a small subfamily of the immunoglobulin superfamily of adhesion receptors with a multitude of physiological functions in vertebrate development and homeostasis. Several members of the JAM family localize at tight junctions of epithelial and endothelial cells where they interact with PDZ domain-containing scaffolding proteins. For some JAM family members, molecular mechanisms have been elaborated through which they regulate cell-cell contact ...
4 CitationsSource
#1Sargeet Kaur (JUIT: Jaypee University of Information Technology)H-Index: 2
#2Harish Changotra (JUIT: Jaypee University of Information Technology)H-Index: 12
Abstract Beclin 1 a yeast Atg6/VPS30 orthologue has a significant role in autophagy process (Macroautophagy) and protein sorting. The function of beclin 1 depends on the interaction with several autophagy-related genes (Atgs) and other proteins during the autophagy process. The role mediated by beclin 1 is controlled by various conditions and factors. Beclin 1 is regulated at the gene and protein levels by different factors. These regulations could subsequently alter the beclin 1 induced autopha...
4 CitationsSource
#1Wen Wang (Kunming Medical University)H-Index: 3
#2Fengrui Zhang (Kunming Medical University)H-Index: 3
Last. Yinglei Miao (Kunming Medical University)H-Index: 10
view all 12 authors...
Abstract UC is a chronic inflammatory disease of the colonic mucosa and lacks effective treatments because of unclear pathogenesis. Excessive apoptosis of IECs damages the intestinal epithelial barrier and is involved in the progression of UC, but the mechanism is unknown. HSPs are important in maintaining homeostasis and regulate apoptosis through the mitochondrial pathway. In our previous studies, HSF2, an important regulator of HSPs, was highly expressed in UC patients and negatively correlat...
6 CitationsSource
#1Wucheng Qi (NCU: Nanchang University)H-Index: 3
#2Xingtao Zhou (NCU: Nanchang University)H-Index: 4
Last. Mingyong Xie (Jiangxi Normal University)H-Index: 71
view all 8 authors...
Abstract Cordyceps sinensis is thought to have anti-cancer effects, but its mechanisms remain elusive. In this study, we aimed to investigate the anti-cancer effect of Cordyceps sinensis polysaccharide (CSP) on human colon cancer cell line (HCT116) and its mechanism. Results indicated that CSP significantly inhibited the proliferation of HCT116 cells, increased autophagy and apoptosis, while blocked autophagy flux and lysosome formation. Further experiments showed that CSP decreased the expressi...
12 CitationsSource
#1Yu Han (HBUT: Hubei University of Technology)H-Index: 3
#2Zhe Cheng (HBUT: Hubei University of Technology)H-Index: 2
Last. Youyi Huang (HAU: Huazhong Agricultural University)H-Index: 1
view all 14 authors...
Abstract Polysaccharide conjugates were water-extracted from low-grade green tea; this extract was designated as TPC-W. Various concentrations of TPC-W (0.5, 1.0, 1.5, 2.0, and 3.0 wt%) were then used as an emulsifier stabilize to oil-in-water emulsions prepared using medium chain triglycerides. The emulsion stabilized using 2.0 wt% TPC-W remained relatively constant during 10 days of storage at 25 °C, with the mean particle diameter (MPD) (d32) and zeta potential increasing from 1.8 to 2.4 μm a...
18 CitationsSource
#1Kai Sun (HUST: Huazhong University of Science and Technology)H-Index: 9
#2Jiahui Luo (HUST: Huazhong University of Science and Technology)H-Index: 2
Last. Fengjing Guo (HUST: Huazhong University of Science and Technology)H-Index: 10
view all 6 authors...
Summary Osteoarthritis (OA) is a complicated degenerative disease that affects whole joint tissue. Currently, apart from surgical approaches to treat late stage OA, effective treatments to reverse OA are not available. Thus, the mechanisms leading to OA, and more effective approaches to treat OA should be investigated. According to available evidence, the PI3K/AKT/mTOR signaling pathway is essential for normal metabolism of joint tissues, but is also involved in development of OA. To provide a w...
25 CitationsSource
#1Xiaomeng Ding (NCU: Nanchang University)H-Index: 3
#2Qiang Yu (NCU: Nanchang University)H-Index: 21
Last. Mingyong Xie (Jiangxi Normal University)H-Index: 71
view all 9 authors...
Abstract Our previous study has demonstrated that Ganoderma atrum polysaccharide (PSG-1) activated the immune response of dendritic cells (DCs) by direct contacting with surface receptors. However, as a kind of macromolecule compound, PSG-1 cannot directly stimulate DCs via passing through the intestine epithelial cells. To investigate the role of PSG-1 in the interaction between the intestinal epithelium and its internal immune cells, the intestinal-like Caco-2/DCs co-culture model was establis...
4 CitationsSource
#1Mengxi Ying (NCU: Nanchang University)H-Index: 3
#2Bing Zheng (NCU: Nanchang University)H-Index: 3
Last. Xie Mingyong (Jiangxi Normal University)H-Index: 4
view all 10 authors...
Abstract The aim of this study was to investigate the protective effect and underlying mechanisms of Ganoderma atrum polysaccharide (PSG-1) on cyclophosphamide (Cy)-induced intestinal mucosal dysfunction in mice. Results showed that PSG-1 promoted the formation of IgA-secreting cells, modulated sIgA, IgE, IgG, IgM secretion, and improved TLR-2, TLR-4, TLR-6 mRNA levels while these factors were suppressed after Cy treatment. CD4+ and CD8+ T cell numbers were also elevated by PSG-1. Cytokines incl...
16 CitationsSource
#1Tang Li (First Affiliated Hospital of Wenzhou Medical University)H-Index: 3
#2Ru-ru Chen (First Affiliated Hospital of Wenzhou Medical University)H-Index: 3
Last. Zhiming Huang (First Affiliated Hospital of Wenzhou Medical University)H-Index: 11
view all 7 authors...
Abstract Inflammatory bowel disease (IBD) is an autoimmune disease characterized by an abnormal immune response. Fibrinogen-like protein 2 (FGL2) is known to have immunoregulatory and anti-inflammatory activity. The level of FGL2 is elevated in patients with IBD; however, its comprehensive function in IBD is almost unknown. In our study, we explored the effect of FGL2 on dextran sulfate sodium (DSS)-induced colitis in mice and on NF-κB signaling in intestinal epithelial cells (IECs) and lamina p...
6 CitationsSource
#1Xingtao Zhou (NCU: Nanchang University)H-Index: 4
#2Ke Zhang (NCU: Nanchang University)H-Index: 5
Last. Shaoping Nie (NCU: Nanchang University)H-Index: 65
view all 8 authors...
Probiotic lactobacilli and their exopolysaccharides (EPS) are thought to modulate mucosal homeostasis; however, their mechanisms remain elusive. Thus, we tried to clarify the role of exopolysaccharides from Lactobacillus plantarum NCU116 (EPS116) in the intestinal mucosal homeostasis. Our results indicated that EPS116 regulated the colon mucosal healing and homeostasis, enhanced the goblet cell differentiation, and promoted the expression of Muc2 gene in vivo and in vitro. Further experiments sh...
10 CitationsSource
Cited By2
#1Chang Liu (Jiangnan University)H-Index: 2
#1Chang Liu (Jiangnan University)H-Index: 4
Last. He Qian (Jiangnan University)H-Index: 21
view all 7 authors...
Abstract null null Aloe polysaccharides (APs) are acetyl polysaccharides. It has been reported APs could protect mice from ulcerative colitis (UC), but the complex interactions between APs and the intestinal barrier were unclear. Here, we investigated the relationship between APs and UC, and determined the synergistic effects of Nrf2/HO-1 signaling pathway and short-chain fatty acids (SCFAs) metabolism on protecting intestinal barrier in acute UC mice. Results showed APs could scavenge free radi...
#1Ya-Gang Song (Henan University)H-Index: 1
#2Le Kang (Henan University)H-Index: 1
Last. Mingsan Miao (Henan University)H-Index: 7
view all 10 authors...
abstract Ethnopharmacological relevance The traditional use of Prunella vulgaris was for the treatment of liver cancer in a few areas of China. At present, it is mainly used in the treatment of thyroid cancer, throat cancer, and lymphosarcoma among others. However there are few current scientific reports of its use in the treatment of liver cancer. We are committed to studying the effective treatment of liver cancer in order to provide an experimental basis for the application of Prunella vulgar...