Sodium alginate/collagen composite multiscale porous scaffolds containing poly(ε-caprolactone) microspheres fabricated based on additive manufacturing technology

Published on Oct 21, 2020in RSC Advances3.119
· DOI :10.1039/D0RA04581K
Liu Shuifeng4
Estimated H-index: 4
,
Da Huang4
Estimated H-index: 4
+ 7 AuthorsWuyi Zhou19
Estimated H-index: 19
Sources
Abstract
Biocompatible porous scaffolds with adjustable pore structures, appropriate mechanical properties and drug loading properties are important components of bone tissue engineering. In this work, biocompatible sodium alginate (SA)/collagen (Col) multiscale porous scaffolds containing poly(e-caprolactone) microspheres (Ms-PCL) have been facilely fabricated based on 3D extrusion printing of the pre-crosslinked composite hydrogels. The prepared composite hydrogels can be 3D extrusion printed into porous scaffolds with different designed shapes and adjustable pore structures. The hydroxyapatite (HAP) nanoparticles have been added into the SA/Col hydrogels to achieve stress dispersion and form double crosslinking networks. SA-Ca2+ crosslinking networks and Col–genipin (GP) crosslinking networks have been constructed to improve the mechanical properties of the scaffolds (about 2557 kPa of compressive stress at 70% strain), and reduce the swelling rate and degradation rate of SA/Col scaffolds. Moreover, the SA/Col hydrogels contain hydrophobic antibacterial drug enrofloxacin loaded Ms-PCL, and in vitro drug release research shows a sustained-release function of porous scaffolds, indicating the potential application of SA/Col porous scaffolds as drug carriers. In addition, the antibacterial experiments show that the composite scaffolds display a distinguished and long-term antibacterial activity against Escherichia coli and Staphylococcus aureus. Furthermore, mouse bone mesenchymal stem cells (mBMSCs) are seeded on the SA/Col composite scaffolds, and an in vitro biocompatibility experiment shows that the mBMSCs can adhere well on the composite scaffolds, which indicate that the fabricated composite scaffolds are biocompatible. In short, all of the above results suggest that the biocompatible SA/Col composite porous scaffolds have enormous application and potential in bone tissue engineering.
References57
Newest
#1Nilabh S. Kajave (FIT: Florida Institute of Technology)H-Index: 1
#2Trevor J. Schmitt (FIT: Florida Institute of Technology)H-Index: 1
Last. Vipuil Kishore (FIT: Florida Institute of Technology)H-Index: 16
view all 4 authors...
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#2M. GobalakrishnanH-Index: 3
Last. M.G. KarthihH-Index: 3
view all 4 authors...
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Last. Hossein Semyari (Shahed University)H-Index: 3
view all 8 authors...
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#1K. N. Bardakova (RAS: Russian Academy of Sciences)H-Index: 6
#2E.A. GrebenikH-Index: 1
Last. Petr Timashev (RAS: Russian Academy of Sciences)H-Index: 9
view all 8 authors...
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#2Jiping Zhou (YZU: Yangzhou University)H-Index: 5
Last. Xiaodong Xv (YZU: Yangzhou University)H-Index: 3
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Last. Xuefen Wang (Donghua University)H-Index: 29
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Collagen hydrogel applications in tissue engineering are limited due to its weak physical and mechanical properties, e.g. loss of water, destruction in the biological medium, weak mechanical properties, and handling difficulty. To improve the physical and mechanical properties of collagen hydrogel, cellulose nanofibers (CNF) were introduced to the collagen hydrogel. Up to 8% CNF, by total dry weight, was added to cold collagen acidic solution and the solution underwent gel formation by increasin...
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#1Shithima Sayed (SCH: Soonchunhyang University)H-Index: 2
#2Omar Faruq (SCH: Soonchunhyang University)H-Index: 4
Last. Byong-Taek Lee (SCH: Soonchunhyang University)H-Index: 35
view all 6 authors...
Abstract The application of heat stress on a defect site during the healing process is a promising technique for early bone regeneration. The primary goal of this study was to investigate the effect of periodic heat shock on bone formation. MC3T3-E1 cells were seeded onto biphasic calcium phosphate (BCP) scaffolds, followed by periodic heating to evaluate osteogenic differentiation. Heat was applied to cells seeded onto scaffolds at 41 °C for 1 h once, twice, and four times a day for seven days ...
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: To successfully achieve the porous cell-blocks, a bioink is a prerequisite requirement. However, although various hydrogel-based bioinks have been applied, a hydrogel/bioceramic-based composite bioink consisting of cells has not been actively investigated owing to its poor printability and low initial cell-viability. In this study, a new bioink consisting of fibrillated collagen, cells, and bioceramic (β-TCP) is suggested to attain a 3D porous cell-laden composite structure with high cellular ...
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