Sodium alginate/collagen composite multiscale porous scaffolds containing poly(ε-caprolactone) microspheres fabricated based on additive manufacturing technology

Published on Oct 21, 2020in RSC Advances3.119
· DOI :10.1039/D0RA04581K
Liu Shuifeng4
Estimated H-index: 4
Da Huang4
Estimated H-index: 4
+ 7 AuthorsWuyi Zhou19
Estimated H-index: 19
Biocompatible porous scaffolds with adjustable pore structures, appropriate mechanical properties and drug loading properties are important components of bone tissue engineering. In this work, biocompatible sodium alginate (SA)/collagen (Col) multiscale porous scaffolds containing poly(e-caprolactone) microspheres (Ms-PCL) have been facilely fabricated based on 3D extrusion printing of the pre-crosslinked composite hydrogels. The prepared composite hydrogels can be 3D extrusion printed into porous scaffolds with different designed shapes and adjustable pore structures. The hydroxyapatite (HAP) nanoparticles have been added into the SA/Col hydrogels to achieve stress dispersion and form double crosslinking networks. SA-Ca2+ crosslinking networks and Col–genipin (GP) crosslinking networks have been constructed to improve the mechanical properties of the scaffolds (about 2557 kPa of compressive stress at 70% strain), and reduce the swelling rate and degradation rate of SA/Col scaffolds. Moreover, the SA/Col hydrogels contain hydrophobic antibacterial drug enrofloxacin loaded Ms-PCL, and in vitro drug release research shows a sustained-release function of porous scaffolds, indicating the potential application of SA/Col porous scaffolds as drug carriers. In addition, the antibacterial experiments show that the composite scaffolds display a distinguished and long-term antibacterial activity against Escherichia coli and Staphylococcus aureus. Furthermore, mouse bone mesenchymal stem cells (mBMSCs) are seeded on the SA/Col composite scaffolds, and an in vitro biocompatibility experiment shows that the mBMSCs can adhere well on the composite scaffolds, which indicate that the fabricated composite scaffolds are biocompatible. In short, all of the above results suggest that the biocompatible SA/Col composite porous scaffolds have enormous application and potential in bone tissue engineering.
#1Nilabh S. Kajave (FIT: Florida Institute of Technology)H-Index: 1
#2Trevor J. Schmitt (FIT: Florida Institute of Technology)H-Index: 1
Last. Vipuil Kishore (FIT: Florida Institute of Technology)H-Index: 16
view all 4 authors...
Abstract Photopolymerization of methacrylated collagen (CMA) allows for 3D bioprinting of tissue scaffolds with high resolution and print fidelity. However, photochemically crosslinked CMA constructs are mechanically weak and susceptible to expedited enzymatic degradation in vivo. The goal of the current study was to develop a dual crosslinking scheme for the generation of mechanically viable cell-laden printable constructs for tissue engineering applications. Dual crosslinking was performed by ...
12 CitationsSource
#1S.R. Radhika Rajasree (Kerala University of Fisheries and Ocean Studies)H-Index: 9
#2M. GobalakrishnanH-Index: 3
Last. M.G. KarthihH-Index: 3
view all 4 authors...
Abstract In this study, for developing a scaffold for tissue engineering from fish processing wastes, a hierachial collagen/gelatin/chitosan novel porous scaffold was fabricated using blends of collagen and gelatin extracted from the skins of Marine big eye snapper Priacanthus humrur. Scaffolds were developed by mechanical spinning of chitosan and by mixing of collagen and gelatin solutions followed by freeze drying and subsequent crosslinking of polymers. The scaffolds were evaluated for rheolo...
9 CitationsSource
#1Mir Hamed Nabavi (Shahed University)H-Index: 2
#2Majid Salehi (Shahroud University of Medical Sciences)H-Index: 20
Last. Hossein Semyari (Shahed University)H-Index: 3
view all 8 authors...
Bone tissue engineering aims to develop bone graft structure that can heal bone defects without using autografts or allografts. The current study was conducted to promote bone regeneration using a collagen type I hydrogel containing tacrolimus. For this purpose, different amounts of tacrolimus (10 μg/ml, 100 μg/ml, and 1000 μg/ml) were loaded into the hydrogel. The resulting drug-loaded hydrogels were characterized for their porosity, swelling capacity, weight loss, drug release, blood compatibi...
7 CitationsSource
#1K. N. Bardakova (RAS: Russian Academy of Sciences)H-Index: 6
#2E.A. GrebenikH-Index: 1
Last. Petr Timashev (RAS: Russian Academy of Sciences)H-Index: 9
view all 8 authors...
Abstract Application of restructured collagen-based biomaterials is generally restricted by their poor mechanical properties, which ideally must be close to those of a tissue being repaired. Here, we present an approach to the formation of a robust biomaterial using laser-induced curing of a photosensitive star-shaped polylactide. The created collagen-based structures demonstrated an increase in the Young's modulus by more than an order of magnitude with introduction of reinforcing patterns (fro...
6 CitationsSource
#1Jiang Yani (YZU: Yangzhou University)H-Index: 5
#2Jiping Zhou (YZU: Yangzhou University)H-Index: 5
Last. Xiaodong Xv (YZU: Yangzhou University)H-Index: 3
view all 7 authors...
Early reports have demonstrated the feasibility of oxidized dextran/gelatin (OD/GEL) hydrogel for tissue engineering applications and the printability of OD/GEL hydrogel for 3D printing scaffolds, but adequate insight into fabricated OD/GEL scaffolds is lacking. In the present study, we prepared (cellulose nanocrystal) CNC/OD/GEL hydrogels and fabricated CNC/OD/GEL scaffolds by 3D printing. The properties of CNC/OD/GEL hydrogels were investigated by scanning electron microscopy (SEM) and Fourier...
13 CitationsSource
#1Juan Chen (Donghua University)H-Index: 1
#2Tonghui Zhang (Donghua University)H-Index: 11
Last. Xuefen Wang (Donghua University)H-Index: 29
view all 5 authors...
Abstract One of the serious challenges in bone tissue engineering is the construction of biomimetic scaffolds, which can bridge the gap between mechanical strength and porous structure requirements. Although electrospinning technology can be used to create nanofibrous networks with a structure of artificial extracellular matrix (ECM), the restricted shapes and pore sizes block its application. Herein, we combine technologies of freeze-drying and crosslinking to fabricate a novel three-dimensiona...
12 CitationsSource
#1Sajedeh Lohrasbi (Shiraz University of Medical Sciences)H-Index: 2
#2Esmaeil Mirzaei (Shiraz University of Medical Sciences)H-Index: 13
Last. Anita Rezaei (Shiraz University of Medical Sciences)H-Index: 1
view all 5 authors...
Collagen hydrogel applications in tissue engineering are limited due to its weak physical and mechanical properties, e.g. loss of water, destruction in the biological medium, weak mechanical properties, and handling difficulty. To improve the physical and mechanical properties of collagen hydrogel, cellulose nanofibers (CNF) were introduced to the collagen hydrogel. Up to 8% CNF, by total dry weight, was added to cold collagen acidic solution and the solution underwent gel formation by increasin...
12 CitationsSource
#1Shithima Sayed (SCH: Soonchunhyang University)H-Index: 2
#2Omar Faruq (SCH: Soonchunhyang University)H-Index: 4
Last. Byong-Taek Lee (SCH: Soonchunhyang University)H-Index: 35
view all 6 authors...
Abstract The application of heat stress on a defect site during the healing process is a promising technique for early bone regeneration. The primary goal of this study was to investigate the effect of periodic heat shock on bone formation. MC3T3-E1 cells were seeded onto biphasic calcium phosphate (BCP) scaffolds, followed by periodic heating to evaluate osteogenic differentiation. Heat was applied to cells seeded onto scaffolds at 41 °C for 1 h once, twice, and four times a day for seven days ...
4 CitationsSource
#1Yannan Liu (Northwest University (United States))H-Index: 1
#2Daidi Fan (Northwest University (United States))H-Index: 1
Last. Daidi Fan (Northwest University (United States))H-Index: 25
view all 2 authors...
Abstract In this study, we fabricated multifunctionalized hydrogel scaffolds based on hyaluronic acid (HA)-tyrosine and human-like collagen (HLC) by crosslinking with 1, 4-butanedioldiglycidyl ether (BDDE) for soft tissue fillers. The physicochemical of HA-tyrosine/BDDE (HTB) and various proportions of HA-tyrosine/BDDE/HLC (HTBH) hydrogels were characterized by swelling ratio, mechanical strength, morphology and thermal stability. The results demonstrated various HTBH hydrogels had superior perf...
11 CitationsSource
#1WonJin Kim (SKKU: Sungkyunkwan University)H-Index: 12
#2GeunHyung KimH-Index: 46
: To successfully achieve the porous cell-blocks, a bioink is a prerequisite requirement. However, although various hydrogel-based bioinks have been applied, a hydrogel/bioceramic-based composite bioink consisting of cells has not been actively investigated owing to its poor printability and low initial cell-viability. In this study, a new bioink consisting of fibrillated collagen, cells, and bioceramic (β-TCP) is suggested to attain a 3D porous cell-laden composite structure with high cellular ...
11 CitationsSource
Cited By1
#1Claire BenwoodH-Index: 1
#2Josie ChrenekH-Index: 1
Last. Stephanie M. Willerth (UVic: University of Victoria)H-Index: 24
view all 7 authors...
The most prevalent form of bioprinting—extrusion bioprinting—can generate structures from a diverse range of materials and viscosities. It can create personalized tissues that aid in drug testing and cancer research when used in combination with natural bioinks. This paper reviews natural bioinks and their properties and functions in hard and soft tissue engineering applications. It discusses agarose, alginate, cellulose, chitosan, collagen, decellularized extracellular matrix, dextran, fibrin, ...
1 CitationsSource