Bead‐Based Extracellular Vesicle Analysis Using Flow Cytometry

Extracellular vesicles (EVs) represent promising circulating biomarkers for cancers, but their high‐throughput analyses in clinical settings prove challenging due to lack of simple, fast, and robust EV assays. Here, a bead‐based EV assay detected by flow cytometry is described, which integrates EV capture using microbeads with EV protein analyses by flow cytometry. The assay is fast (<4 h for 48 samples), robust, and compatible with...