Katherine S. Yang
University of Pittsburgh
Receptor tyrosine kinasePharmacokineticsCancerBiophysicsInternal medicinePhenotypeMolecular imagingDNA damageMolecular biologySmall moleculeReceptorChemistryIn vivoGrowth factor receptorEpidermal growth factorSingle-cell analysisDrug deliveryOlaparibDrugPancreatic cancerPoly (ADP-Ribose) Polymerase InhibitorPreclinical imagingExtracellular vesiclesTyrosine kinaseCancer researchFlow cytometryBiochemistryStereochemistryMedicineCell cultureBiologyBioorthogonal chemistryCell biologyCancer cellPharmacologyDistribution (pharmacology)
36Publications
20H-index
1,546Citations
Publications 36
Newest
#1Somaiah Chinnapaka (University of Pittsburgh)H-Index: 1
#2Katherine S. Yang (University of Pittsburgh)H-Index: 20
Last. J. Peter Rubin (University of Pittsburgh)H-Index: 60
view all 8 authors...
Acute radiation syndrome (ARS) is the radiation toxicity that can affect the hematopoietic, gastrointestinal, and nervous systems upon accidental radiation exposure within a short time. Currently, there are no effective and safe approaches to treat mass population exposure to ARS. Our study aimed to evaluate the therapeutic potential of allogeneic adipose-derived stem cells (ASCs) for total body irradiation (TBI)-induced ARS and understand the underlying mitigation mechanism. We employed 9.25 Gy...
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#1Katherine S. Yang (Harvard University)H-Index: 20
#2Debora Ciprani (Harvard University)H-Index: 4
Last. Ralph Weissleder (Harvard University)H-Index: 205
view all 9 authors...
Background and Aims Advances in cross-sectional imaging have resulted in increased detection of intraductal papillary mucinous neoplasms (IPMNs), and their management remains controversial. At present, there is no reliable noninvasive method to distinguish between indolent and high risk IPMNs. We performed extracellular vesicle (EV) analysis to identify markers of malignancy in an attempt to better stratify these lesions. Methods Using a novel ultrasensitive digital extracellular vesicle screeni...
1 CitationsSource
#1Asim Ejaz (University of Pittsburgh)H-Index: 6
#2Katherine S. Yang (University of Pittsburgh)H-Index: 20
Last. J. Peter Rubin (University of Pittsburgh)H-Index: 60
view all 6 authors...
Background: Autologous fat transfer in the form of lipoaspirates for the reconstruction of the breast after breast cancer surgery is a commonly used procedure in plastic surgery. However, concerns regarding the oncologic risk of nutrient-rich fat tissue are widely debated. Previous studies have primarily focused on studying the interaction between adipose-derived stem cells (ASCs) and breast cancer cells. Methods: In this study, we performed a comprehensive analysis of the paracrine- and contact...
1 CitationsSource
#1Katherine S. Yang (Harvard University)H-Index: 20
#2Hsing-Ying Lin (Harvard University)H-Index: 15
Last. Cesar M. Castro (Harvard University)H-Index: 29
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Extracellular vesicles (EVs) represent promising circulating biomarkers for cancers, but their high-throughput analyses in clinical settings prove challenging due to lack of simple, fast, and robust EV assays. Here, a bead-based EV assay detected by flow cytometry is described, which integrates EV capture using microbeads with EV protein analyses by flow cytometry. The assay is fast (<4 h for 48 samples), robust, and compatible with conventional flow cytometry instruments for high-throughput EV ...
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#1Kyle B. Fraser (Harvard University)H-Index: 15
#1Kyle Fraser (Harvard University)H-Index: 4
Last. Ralph Weissleder (Harvard University)H-Index: 205
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BACKGROUND: Extracellular vesicles (EV) are shed by tumor cells but little is known about their individual molecular phenotypes and heterogeneity. While exosomes have received considerable attention, much less is known about larger microvesicles. Here we profile single microvesicles (MV) and exosomes from glioblastoma (GB) cells and MV from the plasma of patients. METHODS: EV secreted from mouse glioma GL261 and human primary GBM8 cell lines as well as from the plasma of 8 patients with diagnose...
26 CitationsSource
#1Hsing-Ying Lin (Harvard University)H-Index: 15
#2Katherine S. Yang (Harvard University)H-Index: 20
Last. Cesar M. Castro (Harvard University)H-Index: 29
view all 9 authors...
Extracellular vesicles (EVs) are recognized cancer biomarkers, however, clinical analysis has been difficult due to a lack of simple and sensitive assays. Here, we describe a bead-enhanced flow cytometry method, BEAD flow, using biotinylated EVs captured on streptavidin particles. With this method, we show analysis of patient-derived EVs using a panel of pancreatic cancer biomarkers. BEAD flow is easily translatable to any biomarker or cancer type and can be run with conventional flow cytometers...
1 CitationsSource
#1Hyungsoon Im (Harvard University)H-Index: 41
#2Katherine S. Yang (Harvard University)H-Index: 20
Last. Cesar M. Castro (Harvard University)H-Index: 29
view all 4 authors...
Abstract Extracellular vesicles (EVs) or exosomes represent a new opportunity for cancer management through liquid biopsies. EVs carry cell-specific biomarkers, which can be exploited as a minimally invasive means to probe the molecular status of tumors. Despite their clinical potential, simple, reliable and sensitive detection and molecular profiling of EVs has been challenging. Thus, there is a clear need to develop next-generation sensing technologies for sensitive and robust EV detection and...
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#1Katherine S. Yang (Harvard University)H-Index: 20
#2Hyungsoon Im (Harvard University)H-Index: 41
Last. Ralph Weissleder (Harvard University)H-Index: 205
view all 15 authors...
Pancreatic ductal adenocarcinoma (PDAC) is usually detected late in the disease process. Clinical workup through imaging and tissue biopsies is often complex and expensive due to a paucity of reliable biomarkers. We used an advanced multiplexed plasmonic assay to analyze circulating tumor-derived extracellular vesicles (tEVs) in more than 100 clinical populations. Using EV-based protein marker profiling, we identified a signature of five markers (PDAC EV signature) for PDAC detection. In our pro...
102 CitationsSource
#1Sean P. Arlauckas (Harvard University)H-Index: 12
#2Christopher Garris (Harvard University)H-Index: 19
Last. Mikael J. Pittet (Harvard University)H-Index: 81
view all 12 authors...
Monoclonal antibodies (mAbs) targeting the immune checkpoint anti–programmed cell death protein 1 (aPD-1) have demonstrated impressive benefits for the treatment of some cancers; however, these drugs are not always effective, and we still have a limited understanding of the mechanisms that contribute to their efficacy or lack thereof. We used in vivo imaging to uncover the fate and activity of aPD-1 mAbs in real time and at subcellular resolution in mice. We show that aPD-1 mAbs effectively bind...
229 CitationsSource
#1Randy J. Giedt (Harvard University)H-Index: 12
#2Paolo Fumene Feruglio (University of Verona)H-Index: 14
Last. Ralph Weissleder (Harvard University)H-Index: 205
view all 8 authors...
Computational imaging reveals mitochondrial morphology as a biomarker of cancer phenotype and drug response
32 CitationsSource