Simultaneous imaging of lysosomal and mitochondrial viscosity under different conditions using a NIR probe

This article reported a hemicyanine dye fluorescent probe (1), based on benzo[e]indole and aromatic azonia skeleton, for simultaneous detection of viscosity fluctuation in mitochondria and lysosomes. Probe 1 exhibited near infrared emission and high sensitivity towards viscosity of the medium. As the viscosity increases, the rotation of CC single bond between the benzo[e]indole and the nitrogen heterocycle could be suppressed, and the entire...