Preliminary study of icariin indicating prevention of steroid-induced osteonecrosis of femoral head by regulating abnormal expression of miRNA-335 and protecting the functions of bone microvascular endothelial cells in rats

Published on Jan 15, 2021in Gene2.984
· DOI :10.1016/J.GENE.2020.145128
Ju'an Yue3
Estimated H-index: 3
Huachen Yu2
Estimated H-index: 2
(Peking Union Medical College)
+ 4 AuthorsQidong Zhang10
Estimated H-index: 10
(China-Japan Friendship Hospital)
Abstract Background The pathogenesis of osteonecrosis of the femoral head (ONFH) is unclear. Our previous study demonstrated that upregulated miR-335 in bone microvascular endothelial cells (BMECs) might be associated with the disease of steroid-induced ONFH. Here, we study the preventive effect of ICA on steroid-induced ONFH in rats. Method 90 rats were separated into three groups: control group, methylprednisolone (MPS) group, and MPS + Icariin (ICA) group. Four weeks later, histological analyses were performed. Thrombomodulin (TM) and vascular endothelial growth factor (VEGF) were tested. MiRNA-335 expression was screened in the three groups using Agilent Gene Spring GX software. Target genes of miRNA-335 were detected by bioinformatics analysis. The functions of BMECs were analyzed by scratch, angiogenesis and cell survival rate. Results ICA can prevent the occurrence of steroid-associated ONFH in rats and reduce the amount of TM and VEGF in serum induced by glucocorticoids. ICA could regulate the overexpression of miRNA-335 induced by glucocorticoids. We predicted the Gene ontology (GO) and signaling pathways of target genes. At 24 hours, we found that ICA significantly promoted BMECs migration abilities. We also found that ICA could promote the angioplasty ability of BMECs. ICA could improve the survival rate of BMECs after steroid-induced injury. Conclusions ICA is effective to prevent the occurrence of steroidinduced ONFH. ICA has a protective effect against steroid-induced BMECs injury. ICA regulated the imbalance of miRNA-335 expression induced by the glucocorticoid in BMECs, which provides a new viewpoint to explore the mechanism of ICA in preventing steroid-induced ONFH.
📖 Papers frequently viewed together
5 Citations
55 Citations
#1Huachen YuH-Index: 2
#2Ju’an Yue (PRC: China Medical University (PRC))H-Index: 1
Last. Qidong Zhang (China-Japan Friendship Hospital)H-Index: 10
view all 7 authors...
: The injury and dysfunction of the femoral head microvascular endothelial cells are associated with the pathogenesis of glucocorticoid-induced osteonecrosis of the femoral head (ONFH). Reports indicate that icariin (ICA) can enhance vascular roles and also inhibit endothelial cell dysfunction. However, it still remains unclear whether ICA can promote angiogenesis in glucocorticoid-induced ONFH. In this study, we investigate this hypothesis through in vitro and in vivo experiments. Results showe...
5 CitationsSource
#1Qingyu Zhang (China-Japan Friendship Hospital)H-Index: 4
#2Fuqiang Gao (China-Japan Friendship Hospital)H-Index: 12
Last. Zirong Li (China-Japan Friendship Hospital)H-Index: 15
view all 6 authors...
Objective: To evaluate the effects of icariin on autophagy induced by low-concentration of glucocorticoid and exosome production in bone microvascular endothelial cells (BMECs). Methods: BMECs were isolated from femoral heads resected in total hip arthroplasty and then intervened with hydrocortisone of low concentration (0, 0.03, 0.06, 0.10 mg/mL), which were set as groups A, B, C, and D, respectively. On the basis of hydrocortisone intervention, 5×10 -5 mol/L of icariin was added to each group ...
1 CitationsSource
#1Bobin Mi (HUST: Huazhong University of Science and Technology)H-Index: 11
#2Junqing Wang (Ha Tai: Xiamen University)H-Index: 1
Last. Wu Zhou (HUST: Huazhong University of Science and Technology)H-Index: 9
view all 8 authors...
Osteoarthritis (OA) is a common chronic and degenerative joint condition that is mainly characterized by cartilage degradation, osteophyte formation and joint stiffness. The NF-κB signaling pathways in inflammation, autophagy and apoptosis play a prominent role in the progression of OA. Icariin, a prenylated flavonol glycoside extracted from Epimedium, have been proven to exert anti-osteoporotic and anti-inflammatory effects in OA. However, the action mechanisms of its effect on chondrocytes has...
23 CitationsSource
#1Yanwu Hu (JLU: Jilin University)H-Index: 5
#2Hai-Tao Li (Changchun University)H-Index: 2
Last. Zhimin Fan (JLU: Jilin University)H-Index: 1
view all 6 authors...
: Icariin belongs to the family of flavonoids that is extracted from Epimedium brevicornum Maxim, and exhibits antioxidative, antitumorigenic, antiosteoporotic, immunoregulatory and antiatherosclerotic properties. To understand the mechanisms underlying the antiatherosclerotic properties of icariin, the present study investigated the effects of icariin on human vascular endothelial cells (HUVECs) following treatment with oxidized low‑density lipoprotein (ox‑LDL). Thus, following pretreatment wit...
9 CitationsSource
#1Ju'an Yue (Beijing University of Chinese Medicine)H-Index: 3
#2Fuyin Wan (PKU: Peking University)H-Index: 2
Last. Wanshou Guo (China-Japan Friendship Hospital)H-Index: 14
view all 7 authors...
Abstract The study profiled the differential miRNA expression from femoral head bone microvascular endothelial cells (BMECs) between model group and control group to explore the pathogenesis of steroid-induced osteonecrosis of femoral head (ONFH). Twenty 8-week-old Female Sprague-Dawley (SD) rats were randomly divided into control and model groups. Rats in model group received an intraperitoneal injection of 20-μg/kg lipopolysaccharide (LPS) at an interval of 24 h. Then, 24 h later, rats receive...
20 CitationsSource
#1Zengfa Huang (HUST: Huazhong University of Science and Technology)H-Index: 4
#2Cheng Cheng (HUST: Huazhong University of Science and Technology)H-Index: 4
Last. Xiang Wang (HUST: Huazhong University of Science and Technology)H-Index: 2
view all 9 authors...
BACKGROUND/AIMS: Glucocorticoid (GC)-related osteonecrosis of the femoral head (ONFH) is a common complication following administration of steroids to treat many diseases. Our previous study demonstrated that icariin (ICA) might have a beneficial effect on the bone marrow mesenchymal stem cells (BMSCs) of patients with steroid-associated osteonecrosis. In this study, we investigated the underlying mechanisms of ICA associated with the potential enhancement of osteogenesis and anti-adipogenesis i...
20 CitationsSource
#1Yulei Dong (Peking Union Medical College Hospital)H-Index: 5
#2Tao Li (Qingdao University)H-Index: 3
Last. Xisheng Weng (Peking Union Medical College Hospital)H-Index: 20
view all 6 authors...
: The mechanism of steroid-associated femoral head necrosis remains unclear. The present study investigated the role of microRNA-23a-3p (miR-23a-3p) in the incidence of osteonecrosis in a rat model. An miR-23a-3p mimic, an inhibitor and a negative control were transfected into bone mesenchymal stem cells using a lentiviral vector, and then injected into the steroid-induced femoral head necrosis model. Osteonecrosis incidence was assessed by micro computed tomography and histopathology. Low-densi...
8 CitationsSource
#1Jian Fang (Southern Medical University)H-Index: 1
#2Yongjun Zhang (Southern Medical University)H-Index: 1
Icariin is a major bioactive pharmaceutical constituent isolated from Chinese medicine Horny Goat Weed with potent cardiovascular protective functions. Emerging evidence in the past decade has shown that Icariin possesses multiple atheroprotective functions, through multiple mechanisms, including attenuating DNA damage, correcting endothelial dysfunction, inhibiting the proliferation and migration of smooth muscle cells, repressing macrophage-derived foam cell formation and inflammatory response...
32 CitationsSource
#1Biaofang WeiH-Index: 2
#2Wei Wei (Guangzhou University of Chinese Medicine)H-Index: 2
Last. Song LiuH-Index: 1
view all 5 authors...
BACKGROUND AND AIM: The biological functions of non-coding RNAs (ncRNAs) have been widely identified in many human diseases. In the present study, the relationship between long non-coding RNA HOTAIR and microRNA-17-5p (miR-17-5p) and their roles in osteogenic differentiation and proliferation in non-traumatic osteonecrosis of femoral head (ONFH) were investigated. METHODS: The expression levels of HOTAIR and miR-17-5p in the mesenchymal stem cells (MSCs) derived from patients with non-traumatic ...
61 CitationsSource
#1Chenxi Gu (Zhengzhou University)H-Index: 1
#2Yan Xu (Zhengzhou University)H-Index: 4
Last. Guoqiang Zhao (Zhengzhou University)H-Index: 26
view all 9 authors...
miR-27a attenuates adipogenesis and promotes osteogenesis in steroid-induced rat BMSCs by targeting PPARγ and GREM1
52 CitationsSource
Cited By0