Self-Folding 3D Silk Biomaterial Rolls to Facilitate Axon and Bone Regeneration.

Published on Aug 31, 2020in Advanced Healthcare Materials9.933
路 DOI :10.1002/ADHM.202000530
Yimin Huang10
Estimated H-index: 10
(BU: Boston University),
Vincent Fitzpatrick6
Estimated H-index: 6
(Tufts University)
+ 5 AuthorsChen Yang74
Estimated H-index: 74
(BU: Boston University)
Biomaterial scaffold designs are needed for self-organizing features related to tissue formation while also simplifying the fabrication processes involved. Toward this goal, silk protein-based self-folding scaffolds to support 3D cell culture, while providing directional guidance and promotion of cell growth and differentiation, are reported. A simple and robust one-step self-folding approach is developed using bilayers consisting of a hydrogel and silk film in aqueous solution. The 3D silk rolls, with patterns transferred from the initially prepared 2D films, guide the directional outgrowth of neurites and also promote the osteogenic differentiation of human mesenchymal stem cells (hMSCs). The osteogenic outcomes are further supported by enhanced biomechanical performance. By utilizing this self-folding method, cocultures of neurons and hMSCs are achieved by patterning cells on silk films and then converting these materials into a 3D format with rolling, mimicking aspects of the structure of osteons and providing physiologically relevant structures to promote bone regeneration. These results demonstrate the utility of self-folded silk rolls as efficient scaffold systems for tissue regeneration, while exploiting relatively simple 2D designs programmed to form more complex 3D structures.
馃摉 Papers frequently viewed together
7 Authors (Chunxiao Cui, ..., Li-Hsin Han)
#1Tamara B. Aigner (University of Bayreuth)H-Index: 6
#2C. Haynl (University of Bayreuth)H-Index: 1
Last. Thomas ScheibelH-Index: 64
view all 5 authors...
Abstract The current gold standard in peripheral nerve repair is nerve autografts for bridging gaps larger than a centimeter. However, autografts are associated with a low availability and the loss of function at the donor site. Nerve guidance conduits (NGCs) made of biocompatible and biodegradable materials reflect suitable alternatives. Clinically approved NGCs comprise either wraps that are rolled around the loose ends of the nerve or steady-state tubes, however both lack internal guidance st...
#1Maria Rosa Iaquinta (University of Ferrara)H-Index: 6
#2Elisa Mazzoni (University of Ferrara)H-Index: 20
Last. Fernanda Martini (University of Ferrara)H-Index: 7
view all 10 authors...
The regeneration of bone fractures, resulting from trauma, osteoporosis or tumours, is a major problem in our super-aging society. Bone regeneration is one of the main topics of concern in regenerative medicine. In recent years, stem cells have been employed in regenerative medicine with interesting results due to their self-renewal and differentiation capacity. Moreover, stem cells are able to secrete bioactive molecules and regulate the behaviour of other cells in different host tissue. Bone r...
#1Chen-Xi Zheng (Fourth Military Medical University)H-Index: 14
#2Ji Chen (Fourth Military Medical University)H-Index: 8
Last. Yan Jin (Fourth Military Medical University)H-Index: 58
view all 4 authors...
In modern medicine, bone and dental loss and defects are common and widespread morbidities, for which regenerative therapy has shown great promise. Mesenchymal stem cells, obtained from various sources and playing an essential role in organ development and postnatal repair, have exhibited enormous potential for regenerating bone and dental tissue. Currently, mesenchymal stem cells (MSCs)-based bone and dental regeneration mainly includes two strategies: the rescue or mobilization of endogenous M...
Encapsulation of enzymes is often necessary to stabilize them against environmental conditions or to protect them from other harmful enzymes such as proteases. Here, a refillable spatial confinement system was produced using a fully degradable self-rolling biopolymer bilayer. The enzyme containers comprise spider silk and chitosan and enable one-pot reactions in the micro- to milliliter regime by trapping the enzyme inside the semipermeable tube and allow the substrate and/or product either to d...
#1Luzhong Zhang (Brandeis University)H-Index: 5
#2Xin Liu (Nantong University)H-Index: 3
Last. Yumin Yang (Nantong University)H-Index: 32
view all 5 authors...
: In tissue regenerative medicine, developing tunable degradation rate of biomaterials for predictive functional outcomes remains critical. The implanted scaffolds should degrade gradually along with the tissue regeneration, and the optimal degradation rate of scaffold depends on the tissue type to be regenerated. Herein, the tunable degradation rates of silk fibroin (SF) scaffolds were fabricated through controlling dissolution, hydrolyzing conditions, and freeze-drying. The pore size, water ad...
Protein-based biomaterials with innovative and controlled performance are being sought due to their unique characteristics for use in biomedical fields such as neural implants, drug delivery systems, cell-based therapies and soft tissue engineering. Here, we present a versatile approach for the synthesis of photo-crosslinkable fibroin silk biomaterial with highly tunable mechanical, chemical and biodegradation properties. Unlike the crystalline rich silk fibroin reported previously, the covalent...
#1Mehdi Farokhi (Pasteur Institute of Iran)H-Index: 29
#2Fatemeh Mottaghitalab (Tehran University of Medical Sciences)H-Index: 21
Last. David L. Kaplan (Tufts University)H-Index: 199
view all 5 authors...
Recently, biomimetic wound dressings were introduced as potential replacements for treating skin injuries. Although there are some clinically available skin replacements, the range of wound types and locations necessitates a broader range of options for the clinic. Natural polymeric-based dressings are of central interest in this area due to their outstanding biocompatibility, biodegradability, low toxicity, and non-allergenic nature. Among them, silk fibroin (SF) has exceptional characteristics...
#1Vladislav Stroganov (UGA: University of Georgia)H-Index: 3
#2Jitendra Pant (UGA: University of Georgia)H-Index: 14
Last. Leonid Ionov (University of Bayreuth)H-Index: 50
view all 8 authors...
#1Yimin Huang (BU: Boston University)H-Index: 10
#2Ying Jiang (BU: Boston University)H-Index: 4
Last. Chen Yang (BU: Boston University)H-Index: 74
view all 9 authors...
After injuries, axonal regeneration over long distance is challenging due to lack of orientation guidance. Biocompatible scaffolds have been used to mimic the native organization of axons to guide and facilitate axonal regeneration. Those scaffolds are of great importance in achieving functional connections of the nervous system. We have developed a nanoladder scaffold to guide directional outgrowth and facilitate regeneration of axons. The nanoladders, composed of micron-scale stripes and nanos...
#1Iman Noshadi (MIT: Massachusetts Institute of Technology)
Photocrosslinkable materials have been frequently used for constructing soft and biomimetic hydrogels for tissue engineering. Although ultraviolet (UV) light is commonly used for photocrosslinking such materials, its use has been associated with several biosafety concerns such as DNA damage, accelerated aging of tissues, and cancer. Here we report an injectable visible light crosslinked gelatin-based hydrogel for myocardium regeneration. Mechanical characterization revealed that the compressive ...
Cited By5
#1Xiao Hu (Rowan University)H-Index: 44
#2Samuel L. Ricci (Rowan University)
Last. Peter Gawason (Rowan University)
view all 5 authors...
Electrically responsive biomaterials are an important and emerging technology in the fields of biomedical and material sciences. A great deal of research explores the integral role of electrical conduction in normal and diseased cell biology, and material scientists are focusing an even greater amount of attention on natural and hybrid materials as sources of biomaterials which can mimic the properties of cells. This review establishes a summary of those efforts for the latter group, detailing t...
#1Rabia Onbas (陌zmir Institute of Technology)H-Index: 2
#2Ahu Arslan Yildiz (陌zmir Institute of Technology)H-Index: 7
Tunable and reproducible size with high circularity is an important limitation to obtain three-dimensional (3D) cellular structures and spheroids in scaffold free tissue engineering approaches. Her...
#1Nethmi Abeynayake (University of Adelaide)H-Index: 1
#2Agnieszka Arthur (University of Adelaide)H-Index: 12
Last. Stan Gronthos (University of Adelaide)H-Index: 87
view all 3 authors...
Emerging evidence in the literature describes a physical and functional association between the neural and skeletal systems that forms a neuro-osteogenic network. This communication between bone cells and neural tissues within the skeleton is important in facilitating bone skeletal growth, homeostasis and repair. The growth and repair of the skeleton is dependent on correct neural innervation for correct skeletal developmental growth and fracture repair, while pathological conditions such as ost...
#1Pedro Morou莽oH-Index: 16
#2Bahareh AzimiH-Index: 9
Last. Serena DantiH-Index: 26
view all 7 authors...
The applications of tissue engineered constructs have witnessed great advances in the last few years, as advanced fabrication techniques have enabled promising approaches to develop structures and devices for biomedical uses. (Bio-)printing, including both plain material and cell/material printing, offers remarkable advantages and versatility to produce multilateral and cell-laden tissue constructs; however, it has often revealed to be insufficient to fulfill clinical needs. Indeed, three-dimens...
#1Agnieszka Arthur (University of Adelaide)H-Index: 12
#2Stan Gronthos (University of Adelaide)H-Index: 87
There has been an escalation in reports over the last decade examining the efficacy of bone marrow derived mesenchymal stem/stromal cells (BMSC) in bone tissue engineering and regenerative medicine-based applications. The multipotent differentiation potential, myelosupportive capacity, anti-inflammatory and immune-modulatory properties of BMSC underpins their versatile nature as therapeutic agents. This review addresses the current limitations and challenges of exogenous autologous and allogenei...
This website uses cookies.
We use cookies to improve your online experience. By continuing to use our website we assume you agree to the placement of these cookies.
To learn more, you can find in our Privacy Policy.