Development of an antimicrobial and antioxidant hydrogel/nano-electrospun wound dressing

Published on Aug 17, 2020in RSC Advances3.119
· DOI :10.1039/D0RA05935H
Alejandra Romero-Montero3
Estimated H-index: 3
(UNAM: National Autonomous University of Mexico),
Pablo Labra-Vázquez5
Estimated H-index: 5
(UNAM: National Autonomous University of Mexico)
+ 4 AuthorsMiquel Gimeno18
Estimated H-index: 18
(UNAM: National Autonomous University of Mexico)
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Abstract
A nanocomposite based on an antibiotic-loaded hydrogel into a nano-electrospun fibre with antimicrobial and antioxidant capacities is investigated. The material is composed of nanofibres of enzymatic PCL grafted with poly(gallic acid) (PGAL), a recently developed enzyme-mediated hydrophilic polymer that features a multiradical and polyanionic nature in a helicoidal secondary structure. An extensive experimental–theoretical study on the molecular structure and morphological characterizations for this nanocomposite are discussed. The hydrogel network is formed by sodium carboxymethylcellulose (CMC) loaded with the broad-spectrum antibiotic clindamycin. This nano electrospun biomaterial inhibits a strain of Staphylococcus aureus, which is the main cause of nosocomial infections. The SPTT assay demonstrates that PGAL side chains also improve the release rates for this bactericide owing to the crosslinking to the CMC hydrogel matrix. The absence of hemolytic activity and the viability of epithelial cells demonstrates that this nanocomposite has no cytotoxicity.
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#1Alejandra Romero-Montero (UNAM: National Autonomous University of Mexico)H-Index: 3
#2Luis J. del ValleH-Index: 27
Last. Miquel Gimeno (UNAM: National Autonomous University of Mexico)H-Index: 18
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Abstract Enzymatic mediated poly (gallic acid) (PGAL), a stable multiradical polyanion with helicoidal secondary structure and high antioxidant capacity, was successfully grafted to poly(e-caprolactone) (PCL) using UV-photo induction. PCL films were prepared with several levels of roughness and subsequently grafted with PGAL (PCL-g-PGAL). The results on the full characterization of the produced materials by mechanical tests, surface morphology, and topography, thermal and crystallographic analys...
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