Rapid characterization of chemical constituents of Shaoyao Gancao decoction using UHPLC coupled with Fourier transform ion cyclotron resonance mass spectrometry
Shaoyao Gancao decoction (SGD), a well-known Chinese herbal formula, has been used to treat liver injury for a long time. In this study, chemical profiles of SGD were identified using ultra high-performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry (UHPLC-FT-ICR-MS/MS). Liquid chromatography was performed on a C18 column (150 mm × 2.1 mm, 1.8 μm); the mobile phase comprised 0.1% formic acid (A) and acetonitrile (B). We then characterized 73 chemical compounds; the primary constituents in SGD included phenols and monoterpenes (in Paeoniae Radix Alba), triterpene saponins, and flavonoids (in Glycyrrhizae Radix et Rhizoma Praeparata Cum Melle). Thus, this study provides a basis for further study on SGD and is expected to be useful for rapidly characterizing constituents in other traditional Chinese herbal formulations.
#1Yueping Jiang(CSU: Central South University)H-Index: 4
#2Rui Liu(CSU: Central South University)H-Index: 3
Last. Shao Liu(CSU: Central South University)H-Index: 13
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Abstract A Chinese herbal formula of Anti-Renal Interstitial Fibrosis I Decoction (ARIF I) is composed of Eucommia ulmoides Oliv., Scutellaria baicalensis Georgi, and Rheum. officinale Baill., and it has been used for the treatment of chronic kidney disease for many years. However, the comprehensive chemical analysis of the formulated ARIF I has yet to be clarified. In this study, an ultra-high performance liquid chromatography coupled with hybrid quadrupole-orbitrap high resolution mass spectro...
#1Binjun Yan(ZJNU: Zhejiang Chinese Medical University)H-Index: 6
#2Menglan Shen(ZJNU: Zhejiang Chinese Medical University)H-Index: 1
Last. Luping Qin(ZJNU: Zhejiang Chinese Medical University)H-Index: 3
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Abstract Paeoniae Radix Alba (baishao or white peony root) and Paeoniae Radix Rubra (chishao or red peony root) are two highly valuable traditional Chinese medicines (TCMs) usually indicated for painful conditions, menstrual disorders and viral infections. These two TCMs are collectively referred to as shaoyao (Paeoniae Radix) due to their close origins and similar chemical compositions. Modern research indicates that monoterpene glycosides, polyphenols and paeonols are the three main types of c...
#1Lanlan Shan(Tianjin University of Traditional Chinese Medicine)H-Index: 3
#2Na Yang(Tianjin University of Traditional Chinese Medicine)H-Index: 1
Last. Yubo Li(Tianjin University of Traditional Chinese Medicine)H-Index: 13
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: Bupleuri radix and liquorice are commonly used as a hepatoprotectants. Their main effective ingredients are triterpenoid saponins. It is known that the saponins in liquorice and Bupleuri radix not only promote the metabolism of sugar and lipids but also have anti-inflammatory functions and hepatoprotective effect. However, the complexity of these compounds results in difficulty in studying their pharmacodynamics and mechanism. In this study, glycosides were the main components that were identi...
Last. Zhiguo Yu(SPU: Shenyang Pharmaceutical University)H-Index: 16
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Abstract Incompatible herb pair Gansui-Gancao is recorded in “eighteen incompatible” medicaments in many monographs of TCM (Traditional Chinese Medicine) which means the two herbs can not be co-used in most cases. However, Gansuibanxia decoction composed of Gansui(Kansui), Banxia(Pinellia), Shaoyao(Peony) and Gancao(Liquorice) is a traditional Chinese formula which has been clinically employed for the treatment of cancerous ascites, pleural effusion, peritoneal effusion, etc. The purpose of the ...
#1Christian Schmid(TUM: Technische Universität München)H-Index: 7
#2Corinna Dawid(TUM: Technische Universität München)H-Index: 16
Last. Thomas Hofmann(TUM: Technische Universität München)H-Index: 42
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European licorice roots (Glycyrrhiza glabra), used in the food and beverage industry due to their distinctive sweet and typical licorice flavor, were fractionated, with the triterpenoid saponins isolated and their chemical structures determined by means of ESIMS, ESIMS/MS, HRESIMS, and 1D/2D NMR experiments. Next to the quantitatively predominant saponin glycyrrhizin (11) and some previously known saponins, the structures of 10 monodesmosidic saponins were assigned unequivocally for the first ti...
Last. Chunjie Zhao(SPU: Shenyang Pharmaceutical University)H-Index: 6
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Abstract Sijunzi decoction, a renowned Chinese prescription has long been utilized to treat gastrointestinal problems. In the context of this research work, the use of Ultra high performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry was made to separate and characterize the components of Sijunzi decoction. The performance of Liquid chromatography was carried out on a C8 column (150 mm × 2.1 mm, 1.8 μm); moreover, the mobile phase were consist...
#1Yangyang Xu(Nanjing University of Chinese Medicine)H-Index: 4
#2Hao Cai(Nanjing University of Chinese Medicine)H-Index: 21
Last. Lin Shen(Nanjing University of Chinese Medicine)H-Index: 3
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Abstract Baizhu Shaoyao San (BSS) is a famous traditional Chinese medicinal formula widely used for the treatment of painful diarrhea, intestinal inflammation, and diarrhea-predominant irritable bowel syndrome. According to clinical medication, three medicinal herbs (Atractylodis Macrocephalae Rhizoma, Paeoniae Radix Alba, and Citri Reticulatae Pericarpium) included in BSS must be processed using some specific methods of stir-frying. On the basis of the classical theories of traditional Chinese ...
#1Azadeh Manayi(Tehran University of Medical Sciences)H-Index: 19
#2Sahar Omidpanah(Tehran University of Medical Sciences)H-Index: 2
Last. Seyed Mohammad Nabavi(BMSU: Baqiyatallah University of Medical Sciences)H-Index: 71
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Paeoniflorin is a monoterpene glycoside, the β-glucoside of paeoniflorigenin. It is one of the major components of Radix Paeoniae, the herbal product obtained from Paeonia lactiflora Pall., a medicinal plant used in folk medicine, especially in Chinese Traditional Medicine, to treat several human ailments, including dementia and cognitive decline. The neuroprotective effects of paeoniflorin have been studied by many investigations showing that its beneficial effects may derive from its anti-infl...
#1Zehua Li(Northwest University (United States))H-Index: 1
#2Haiyang Gao(Northwest University (United States))H-Index: 1
Last. Yajun Zhang(Northwest University (United States))H-Index: 1
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Shaoyao-Gancao decoction, a Chinese herbal formula, is composed of Paeoniae Radix alba and Glycyrrhiza Radix et rhizoma. It has been widely used to treat muscle spasms and asthma. However, little is known about the bioactive components of Shaoyao-Gancao decoction. In the present study, the bioactive compounds in water-extract of Shaoyao-Gancao decoction were separated by the immobilized β2 -adrenoceptor affinity column and identified using quadrupole time-of-flight mass spectrometry. The affinit...
Last. Chunjie Zhao(SPU: Shenyang Pharmaceutical University)H-Index: 5
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Cortex Fraxini is an important traditional Chinese medicine. In this work, a rapid and reliable homogenate extraction method was applied for the fast extraction for Cortex Fraxini, and the method was optimized by response surface methodology. Ultra high performance liquid chromatography combined with Fourier transform ion cyclotron resonance mass spectrometry and gas chromatography with mass spectrometry were established for the separation and characterization of the constituents of Cortex Fraxi...
#2Changliang Yao(CAS: Chinese Academy of Sciences)H-Index: 17
Last. De-An Guo(CAS: Chinese Academy of Sciences)H-Index: 68
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Authentication of Chinese medicine materials in prescriptions is extremely difficult due to the complicated chemical matrix. A strategy integrating in-depth profiling, chemical marker selection, and selected detection was established and exemplarily applied to authenticate paeony root in ShaoYao-GanCao decoction. Firstly, an ultra-performance LC/linear trap quadrupole-Orbitrap method was developed to probe the chemical compositions of the decoction. Secondly, 20 batches of decoctions prepared fr...