Transcriptome Analysis of Nitrogen Metabolism, Transcription Factors, and Indigoid Biosynthesis in Isatis indigotica Fort. Response to Nitrogen Availability

Isatis indigotica Fort. is a popular herb in traditional Chinese medicine. It possesses pharmacological activities against various diseases, particularly leukemia. Indigoid alkaloids are its main bioactive ingredients. A relatively low nitrogen supply increases indigoid content in I. indigotica leaves, yet the mechanisms that regulate indigoid biosynthesis are unknown. To uncover regulatory mechanisms, we performed transcriptome sequencing of...