TET3 overexpression facilitates DNA reprogramming and early development of bovine SCNT embryos

Somatic cell nuclear transfer (SCNT) has been successfully used for cloning in a variety of mammalian species. However, SCNT reprogramming efficiency is relatively low, in part, due to incomplete DNA methylation reprogramming of donor cell nuclei. We previously showed that ten-eleven translocation 3 ( TET3 ) is responsible for active DNA demethylation during preimplantation embryonic development in bovines. In this study, we constructed TET3...