Prediction of the sequence-specific cleavage activity of Cas9 variants

Volume: 38, Issue: 11, Pages: 1328 - 1336
Published: Jun 8, 2020
Abstract
Several Streptococcus pyogenes Cas9 (SpCas9) variants have been developed to improve an enzyme’s specificity or to alter or broaden its protospacer-adjacent motif (PAM) compatibility, but selecting the optimal variant for a given target sequence and application remains difficult. To build computational models to predict the sequence-specific activity of 13 SpCas9 variants, we first assessed their cleavage efficiency at 26,891 target sequences....
Paper Details
Title
Prediction of the sequence-specific cleavage activity of Cas9 variants
Published Date
Jun 8, 2020
Volume
38
Issue
11
Pages
1328 - 1336
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