Purpose To investigate the differential expression of cytokines and growth factors in the cornea and aqueous humor after small incision lenticule extraction (SMILE) compared with femtosecond LASIK (FS-LASIK) using rabbit model. Methods Sixteen eyes of 16 rabbits in each group underwent SMILE or FS-LASIK with refractive correction of -6.00 DS/-1.00 DC. Eight additional rabbits served as controls. Pre- and 24 hours, 1 week, 1 month, and 3 months postoperatively, slit-lamp and anterior segment optical coherence tomography were performed, followed by cornea and aqueous humor collection. Apoptosis and proliferation were evaluated with TUNEL assay and Ki-67 immunostaining, respectively. The mRNA and protein expression of cytokines and growth factors was determined by RT-qPCR and Western blotting, respectively. Cytokine levels in the aqueous humor were detected with ELISA. Results Compared with FS-LASIK, SMILE induced less apoptosis and proliferation in the cornea within 1 week postoperatively. Levels of IL-1β, TNF-α, and EGFR in the cornea were significantly increased after FS-LASIK compared with SMILE within 24 hours. Levels of IL-8 in the aqueous humor remained elevated until 1 week after FS-LASIK but not SMILE. TGF-β1 level was elevated up to 1 month after both procedures, while BFGF level was kept high within 1 month after SMILE but not FS-LASIK. Conclusions SMILE could induce significantly less acute inflammation than FS-LASIK in the cornea and aqueous humor. The differential expression of TGF-β1 and BFGF between two procedures until 1 month might contribute to the post-SMILE delayed recovery and underline the importance of continued treatment postoperatively.
Last. Quan Liu(SYSU: Sun Yat-sen University)H-Index: 10
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PURPOSE: To evaluate the corneal wound healing response after small incision lenticule extraction surgery. METHODS: Small incision lenticule extraction was performed in both eyes of 12 New Zealand White rabbits. The refractive spherical correction was set at -6.00 D. Two animals were analyzed at each time point (1 hour, 4 hours, 1 day, 3 days, 7 days, and 28 days). The corneas were evaluated using slit-lamp and in vivo confocal microscopy. After euthanatization, the corneal tissues were subjecte...
#1Yumi Tsz-Ying Lau(Li Ka Shing Faculty of Medicine, University of Hong Kong)H-Index: 2
#2Kendrick Co Shih(Li Ka Shing Faculty of Medicine, University of Hong Kong)H-Index: 11
Last. Vishal Jhanji(University of Pittsburgh)H-Index: 32
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Laser-assisted in situ keratomileusis (LASIK) is the most commonly performed laser refractive surgical technique worldwide for the treatment of myopia and myopic astigmatism. In recent years, small incision lenticule extraction (SMILE) has emerged as a promising alternative to LASIK, requiring only a single femtosecond laser to create an intrastromal lenticule, which is then removed via a small incision. The technique obviates the need for a corneal flap. A number of published studies have compa...
Last. Xuemin Li(PKU: Peking University)H-Index: 11
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BACKGROUND: To investigate the transparency, biocompatibility, and safety of human-derived acellular dermal matrix for application in corneal stromal transplantation. METHODS: Twenty-four patients (24 eyes) with pellucid marginal corneal degeneration were enrolled, and intrastromal keratoplasty was performed with human-derived acellular dermal matrix. The ocular symptoms and signs as well as graft characteristics were evaluated at baseline and at 1 day, 1 week, and 1, 3, and 6 months postoperati...
PURPOSE: To compare visual outcomes and aberration outcomes in small incision lenticule extraction (SMILE) versus femtosecond laser LASIK (FS-LASIK). METHODS: This prospective, comparative, nonrandomized clinical study included 68 eyes of 37 patients receiving SMILE and 55 eyes of 30 patients receiving FS-LASIK between December 2011 and January 2013 at the Fudan University Eye and ENT Hospital (Shanghai, People's Republic of China). Patients were followed up at 3, 6 months and 5 years after surg...
Small incision lenticule extraction (SMILE) and femtosecond laser-assisted in situ keratomileusis (FS-LASIK) are frequently used to treat myopia. However, little is known about the impact on recovery of these approaches in the very early postsurgical phase (within 24 h). To compare the efficacy of these two procedures for the treatment of myopia in the early phase after surgery, differences in visual acuity, OSI (objective scattering index), cutoff for modulation transfer function (MTF), and SR ...
PURPOSE: To investigate the incidence and clinical results of corneal complications after small incision lenticule extraction (SMILE). METHODS: A retrospective cohort study including 3,223 patients (6,373 eyes) who were treated for myopia or myopic astigmatism was conducted. Postoperative corneal complications were recorded. Postoperative follow-up visits were scheduled on days 1 and 7 and months 1, 3, 6, and 12. RESULTS: Of the 6,373 cases, 432 eyes (6.8%) developed at least one corneal complic...
Abstract Biological materials derived from the extracellular matrix (ECM) of tissues serve as scaffolds for rebuilding tissues and for improved wound healing. Cornea trauma represents a wound healing challenge as the default repair pathway can result in fibrosis and scar formation that limit vision. Effective treatments are needed to reduce inflammation, promote tissue repair, and retain the tissue’s native transparency and vision capacity. Tissue microparticles derived from cornea, cartilage an...
Last. Xiuyun Zheng(Tianjin Medical University)H-Index: 3
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Purpose To compare the effective optical zone (EOZ) after small-incision lenticule extraction (SMILE) and femtosecond laser–assisted laser in situ keratomileusis (FS-LASIK). Setting Jinan Mingshui Eye Hospital, Jinan, China. Design Retrospective case series. Methods Myopic patients who had small-incision lenticule extraction or FS-LASIK were enrolled in this retrospective study. Effective OZs were measured at 1 week, 1 month, and 3 months postoperatively, using the tangential curvature differenc...
Last. Jesper Hjortdal(Aarhus University Hospital)H-Index: 49
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Worldwide, femtosecond Laser Assisted In-situ Keratomileusis (LASIK) is a well known and commonly used refractive technique, although Small Incision Lenticule Extraction (SMILE) has become increasingly popular since it was introduced in 2011. In LASIK, a corneal flap is cut with a microkeratome or femtosecond laser, followed by thinning of the stromal bed with excimer laser ablation. In SMILE, a minor intrastromal lenticule is cut with a femtosecond laser and subsequently removed through a small...
Last. Yan Wang(Tianjin Medical University)H-Index: 16
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ABSTRACTPurpose: To investigate real-time intraocular pressure (IOP) during small incision lenticule extraction (SMILE) in rabbit eyes for myopia correction.Methods: During SMILE, real-time IOP was measured in the vitreous cavity of rabbit eyes with an optic fiber pressure sensor (OFPS). Two groups (n = 6 for each) underwent surgery, one group for a −2.00 diopter (D) refractive spherical correction and the other for a −6.00 D correction.Results: During surgery, the IOP increased once the glass c...
BACKGROUND Spinal cord injury (SCI) is a disabling disorder, resulting in neurological impairments. This study investigated the mechanism of methyltransferase-like 14 (Mettl14) on apoptosis of spinal cord neurons during SCI repair by mediating pri-microRNA (miR) dependent N6-methyladenosine (m6A) methylation. METHODS The m6A content in total RNA and Mettl14 levels in spinal cord tissues of SCI rats were detected. Mettl14 expression was intervened in SCI rats to examine motor function, neuron apo...
Purpose to compare single-step transepithelial PRK (TPRK) to conventional alcohol assisted epithelial removal then PRK (AAPRK) regarding pain, epithelial healing, visual acuity, corneal haze measured subjectively and objectively, higher order aberrations changes, contrast sensitivity and vector analysis of astigmatic correction with one year follow-up. Patients and methods a prospective double-blind randomized study of 29 subjects (58 eyes) who underwent myopic aberration-free laser correction b...