Inhibit NLRC5 regulate CD4+ T cellsfunctionandimmunological protection onallograftinmice

Objective To study the effect of inhibiting NLRC5 on CD4+ T cells function and allografts immune protective effects in mice. Methods Mice spleen-derived CD4+ T cells were cultured, purified andtransfected with short hairpin RNA (shRNA) lentiviral vector NLRC5-RNA interference (RNAi)-green fluorescent protein (GFP). The mice allogenic islet transplantation model andskintransplantation model were established, and 24 h prior to...