Chinese journal of experimental surgery
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Objective To study the mechanism of CC chemokine ligand-5 promoting macrophage infiltration in early stage of hepatic ischemia-reperfusion injury. Methods The adult male C57BL/6 wild type and CC chemokine ligand-5 (CCL5) deficient mice were used for the experiment. The mice were kept in captivity at (23±2) ℃, and were exposed to light and dark cycles for 12 hours to obtain food and water freely. The mice were randomly divided into 4 groups. Sham operation group (sham operation group mice experie...
#1Xiu-Yan Huang (SJTU: Shanghai Jiao Tong University)H-Index: 10
#2Zi-Li Huang (Fudan University)H-Index: 4
Last. Zhao-You Tang (Fudan University)H-Index: 83
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Objective To observe the effects of exosomal circular RNA (circRNA)-100338 derived from hepatocellular carcinoma (HCC) on angiogenesis of human umbilical vein endothelial cells (HUVEC). Methods The exosomes from the supernatant of MHCC97H cells were extracted, purified and identified, and co-incubated with HUVECs (37 ℃, 5% CO2). The exosome tracer experiments traced whether the exosomes could be transformed into HUVECs. MHCC97H cells were transfected with Lipofectamine™ 2000 transfection reagent...
#1Shengzhuang Yang (Guangxi Medical University)
#2Xiangsen Liang (Guangxi Medical University)H-Index: 1
Last. Lei Xian (Guangxi Medical University)H-Index: 7
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#1Zhaohui Hua (Zhengzhou University)H-Index: 3
#2Gaopo Cai (Zhengzhou University)H-Index: 1
Last. Zhen Li (Zhengzhou University)H-Index: 10
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Objective To analyze the correlation between mitochondrial succinate dehydrogenase (SDH) subunit (A, B, C, D) gene mutations and clinicopathological features of carotid body tumor (CBT) and to explore its prognostic value. Methods Ninety-five patients with sporadic CBT (CBT group) (male 43/female 52), aged 39-70 years old, were all unilateral tumors (55 left/40 right), with tumor diameter of 1.5-10.0 cm. Glenner’s classification was as follows: chemoreceptor tumor in 47 cases, pheochromocytoma i...
#1Wenqu Jiang (NCU: Nanchang University)
#2Raorao Yuan (NCU: Nanchang University)H-Index: 2
Last. Yan Zhang (NCU: Nanchang University)H-Index: 4
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Objective To study the expression characteristics of polycomb repressive complex 1 (PRC1) gene in glioma and its influence on the survival of patients, as well as the influence on glioma cells after regulating its expression in vitro, and to evaluate the clinical significance of PRC1 in glioma. Methods Through TCGA database glioma database mining PRC1 mRNA level in glioblastoma tumor tissue, the level of the differences between different level of the WHO, the types of cases, 56 cases of glioma t...
#1Yaozong Yan (Xinxiang Medical University)
#2Bao-zhe Jin (Xinxiang Medical University)
Last. Dainan Zhang (Xinxiang Medical University)
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Objective To observe the effects of neural stem cells (NSCs) combined with neurotrophin-3 gene modified olfactory ensheathing cells (OECs-NT-3) co-transplants on functional recovery after traumatic brain injury in the adult rats. Methods NSCs and OECs were cultured from hippocampus and olfactory bulb of neonatal rats and then identified. OECs-NT-3 was constructed by lentiviral vector containing NT-3 gene. Male SD rats weighing (200±20) g were randomly divided into sham group, model group, OECs-N...
#2Xinguo LiuH-Index: 1
Last. Liang Wang (Zhengzhou University)
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Objective To investigate the effect of RNA binding protein 6 (RBM6) on the proliferation, migration and apoptosis of laryngeal cancer cells. Methods The expression of RBM6 protein was analyzed by Western blotting in 47 cases of laryngeal cancer and adjacent tissues. RBM6 overexpression cell line and control cell line (RBM6 group and control group) were constructed by RBM6 overexpression lentivirus and control lentivirus respectively. The proliferation of two groups was analyzed by cell counting ...
#1Xuzhi Zhang (SYSU: Sun Yat-sen University)H-Index: 4
#2Ao Ren (SYSU: Sun Yat-sen University)H-Index: 2
Last. Yi Ma (SYSU: Sun Yat-sen University)H-Index: 2
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Objective To study the effect of inhibiting NLRC5 on CD4+ T cells function and allografts immune protective effects in mice. Methods Mice spleen-derived CD4+ T cells were cultured, purified andtransfected with short hairpin RNA (shRNA) lentiviral vector NLRC5-RNA interference (RNAi)-green fluorescent protein (GFP). The mice allogenic islet transplantation model andskintransplantation model were established, and 24 h prior to transplantation each recipient mice was given CD4+ T cells, NLRC5-RNAi-...
#1Jianbing Chen (Changzhi Medical College)
#2Fang Wang (Changzhi Medical College)
Last. Jianjun Wu (Changzhi Medical College)
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#1Kaijie Wang (WHU: Wuhan University)H-Index: 2
#2Minglin Zhu (WHU: Wuhan University)H-Index: 4
Last. Jinping Zhao (WHU: Wuhan University)H-Index: 8
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Top fields of study
Molecular biology
Cancer research