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Original paper

Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors

Volume: 17, Issue: 6, Pages: 636 - 642
Published: May 11, 2020
Abstract
Genetic screens using pooled CRISPR-based approaches are scalable and inexpensive, but restricted to standard readouts, including survival, proliferation and sortable markers. However, many biologically relevant cell states involve cellular and subcellular changes that are only accessible by microscopic visualization, and are currently impossible to screen with pooled methods. Here we combine pooled CRISPR–Cas9 screening with microraft array...
Paper Details
Title
Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors
Published Date
May 11, 2020
Volume
17
Issue
6
Pages
636 - 642
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